Influence of IL13 on Periostin Secretion by Synoviocytes in Osteoarthritis
| Autor: | Yasuaki Kanada, Tatsuya Moue, Tadashi Hisamitsu, Yutaro Tajika, Takayuki Okumo, Kazuhito Asano, Shintaro Ishikawa |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research medicine.medical_specialty Enzyme-Linked Immunosorbent Assay Periostin Real-Time Polymerase Chain Reaction General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Internal medicine Osteoarthritis medicine Humans Synovial fluid Secretion Cells Cultured Dexamethasone Leflunomide STAT6 Pharmacology Interleukin-13 Reverse Transcriptase Polymerase Chain Reaction Chemistry Synoviocytes 030104 developmental biology Endocrinology Gene Expression Regulation Interleukin 13 STAT protein STAT6 Transcription Factor Cell Adhesion Molecules Research Article medicine.drug |
| Zdroj: | In Vivo. 31:79-86 |
| ISSN: | 1791-7549 |
| DOI: | 10.21873/invivo.11028 |
| Popis: | Background Our previous research provided evidence of periostin increase in parallel with interleukin-13 (IL13) increase in the synovial fluid of patients with osteoarthritis (OA). The reaction cascade from IL13 to periostin, however, remains unidentified. We, therefore, tested the hypothesis that periostin secretion is affected downstream of IL13. Materials and methods OA synoviocytes were cultured under different concentrations of IL13. Periostin content in culture supernatants and the level of signal transducer and activator of transcription 6 (STAT6) in the cultured cells were measured using enzyme-linked immunosorbent assay (ELISA). Moreover, the influence of dexamethasone and leflunomide on periostin production in relation to the effect of IL13 on the cells was also examined. Results Periostin content in culture supernatants and the level of STAT6 in cultured cells were significantly increased by IL13. The increase of periostin was significantly inhibited by dexamethasone and leflunomide. Conclusion Periostin may be up-regulated in OA synoviocytes via STAT6 downstream of IL13. |
| Databáze: | OpenAIRE |
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