Decellularized and matured esophageal scaffold for circumferential esophagus replacement: Proof of concept in a pig model
| Autor: | Johanna Rannou, Denis Collet, Marlène Durand, Caroline Gronnier, Magali Cabau, Mallory Meulle, Samantha Roques, Guillaume Luc, Reine Bareille, Laurence Bordenave, Guillaume Charles, Lionel Couraud, Charlotte Kalisky |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Scaffold Pathology medicine.medical_specialty Cell Survival Swine Biophysics Biocompatible Materials Bioengineering Proof of Concept Study Biomaterials Rats Nude 03 medical and health sciences Esophagus 0302 clinical medicine Cell Adhesion medicine Animals Humans Rats Wistar Cell Proliferation Basement membrane Decellularization Tissue Engineering Tissue Scaffolds business.industry Stem Cells Cell Differentiation Pig model DNA Prostheses and Implants Stromal vascular fraction Biomechanical Phenomena Extracellular Matrix Colonic interposition 030104 developmental biology medicine.anatomical_structure Adipose Tissue Mechanics of Materials 030220 oncology & carcinogenesis Ceramics and Composites Stem cell business |
| Zdroj: | Biomaterials. 175:1-18 |
| ISSN: | 0142-9612 |
| Popis: | Surgical resection of the esophagus requires sacrificing a long portion of it. Its replacement by the demanding gastric pull-up or colonic interposition techniques may be avoided by using short biologic scaffolds composed of decellularized matrix (DM). The aim of this study was to prepare, characterize, and assess the in vivo remodeling of DM and its clinical impact in a preclinical model. A dynamic chemical and enzymatic decellularization protocol of porcine esophagus was set up and optimized. The resulting DM was mechanically and biologically characterized by DNA quantification, histology, and histomorphometry techniques. Then, in vitro and in vivo tests were performed, such as DM recellularization with human or porcine adipose-derived stem cells, or porcine stromal vascular fraction, and maturation in rat omentum. Finally, the DM, matured or not, was implanted as a 5-cm-long esophagus substitute in an esophagectomized pig model . The developed protocol for esophageal DM fulfilled previously established criteria of decellularization and resulted in a scaffold that maintained important biologic components and an ultrastructure consistent with a basement membrane complex. In vivo implantation was compatible with life without major clinical complications. The DM's scaffold in vitro characteristics and in vivo implantation showed a pattern of constructive remodeling mimicking major native esophageal characteristics. |
| Databáze: | OpenAIRE |
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