Transcriptome, proteome and draft genome of Euglena gracilis

Autor: Steven L. Kelly, Vladimír Hampl, Nithya Vadakedath, Julius Lukeš, Nerissa N. Nankissoor, Mark Carrington, Andrew P. Jackson, Samson O. Obado, Petr Soukal, Anna Nenarokova, Michael Lebert, Damien P. Devos, Anna M. G. Novák Vanclová, Joel B. Dacks, ThankGod E. Ebenezer, Sue Vaughan, Binod Prasad, Sara Silva-Pereira, Alana Burrell, Viktor Daiker, Martin Zoltner, Carlos Santana-Molina, Mark C. Field, Michael L. Ginger, Ellis C. O’Neill
Přispěvatelé: Cambridge Commonwealth European and International Trust, University of Cambridge, Medical Research Council (UK), Federal Ministry of Education and Research (Germany), European Research Council, Ministry of Education, Youth and Sports (Czech Republic), Czech Science Foundation, Apollo - University of Cambridge Repository, Field, Mark C [0000-0002-4866-2885]
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
BMC Biology, Vol 17, Iss 1, Pp 1-23 (2019)
BMC Biology
Popis: © The Author(s) 2019 .
[Background]: Photosynthetic euglenids are major contributors to fresh water ecosystems. Euglena gracilis in particular has noted metabolic flexibility, reflected by an ability to thrive in a range of harsh environments. E. gracilis has been a popular model organism and of considerable biotechnological interest, but the absence of a gene catalogue has hampered both basic research and translational efforts.
[Results]: We report a detailed transcriptome and partial genome for E. gracilis Z1. The nuclear genome is estimated to be around 500 Mb in size, and the transcriptome encodes over 36,000 proteins and the genome possesses less than 1% coding sequence. Annotation of coding sequences indicates a highly sophisticated endomembrane system, RNA processing mechanisms and nuclear genome contributions from several photosynthetic lineages. Multiple gene families, including likely signal transduction components, have been massively expanded. Alterations in protein abundance are controlled post-transcriptionally between light and dark conditions, surprisingly similar to trypanosomatids.
[Conclusions]: Our data provide evidence that a range of photosynthetic eukaryotes contributed to the Euglena nuclear genome, evidence in support of the ‘shopping bag’ hypothesis for plastid acquisition. We also suggest that euglenids possess unique regulatory mechanisms for achieving extreme adaptability, through mechanisms of paralog expansion and gene acquisition.
This work was supported by the Yousef Jameel Academic Program (through the Yousef Jameel PhD Scholarship), the Cambridge Commonwealth, European and International Trust, the Cambridge University Student Registry, the Cambridge Philosophical Society (all to TEE), the Medical Research Council (Grant #: P009018/1 to MCF), and German Aerospace Center - DLR, Cologne, on the behalf of Federal Ministry of Education and Research (BMBF), Germany (Grant no: 50WB1128 and 50WB1528 to ML), the European Research Council CZ LL1601 BFU2013-40866-P (to DPD) and the Czech Ministry of Education, Youth and Sports - National Sustainability Program II (Project BIOCEV-FAR) LQ 1604, by the project BIOCEV (CZ.1.05/1.1.00/02.0109), by the Centre for research of pathogenicity and virulence of parasites CZ.02.1.01/0.0/0.0/16_019/0000759 and by the Czech Science Foundation project nr. 16-25280S (to VH, AV and PS).
Databáze: OpenAIRE
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