Antifungal Effect and Protective Role of Ursolic Acid and Three Phenolic Derivatives in the Management of Sorghum Grain Mold Under Field Conditions
| Autor: | Ravi Sojitra, Mahibalan Senthi, Rukaiyya Khan, Santhosh S. Kumar, Asalla Srinivas, Ragavendra Govardhanam, Sajeli Begum Ahil, Ameer Basha Shaik |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Fusarium Antifungal Agents Bioengineering Microbial Sensitivity Tests 01 natural sciences Biochemistry Alternaria alternata Structure-Activity Relationship 03 medical and health sciences chemistry.chemical_compound Ascomycota Phenols Ursolic acid Botany Methyl caffeate Spore germination Humans Food science Molecular Biology Sorghum Cell Proliferation Dose-Response Relationship Drug Molecular Structure biology Fungi Alternaria food and beverages General Chemistry General Medicine Syringic acid biology.organism_classification Triterpenes HEK293 Cells 030104 developmental biology chemistry Molecular Medicine 010606 plant biology & botany |
| Zdroj: | Chemistry & Biodiversity. 13:1158-1164 |
| ISSN: | 1612-1872 |
| Popis: | In this study, investigation was carried out under in vitro as well as field conditions to explore inhibitors of sorghum grain mold. Phytochemicals, viz., methyl trans-p-coumarate (AIC-1), methyl caffeate (AIC-2), syringic acid (AIC-3), and ursolic acid (UA), at different concentrations (500, 750, and 1000 ppm) were tested on spore germination of Alternaria alternata, Curvularia lunata, Fusarium moniliforme, F. pallidoroseum, and Helminthosporium sp. Significant growth inhibition (P < 0.001) was observed against all fungi except A. alternata which was found to be resistant to AIC-3. Further, two separate sets of field experiments involving spraying of water and F. moniliforme suspension over chemicals treated (1000 ppm) sorghum panicles were done. The levels of protection varied with different treatments which were graded using a standard 1 - 9 rating scale. The Fusarium-challenged panicles (FCP) showed lesser susceptibility and decreased the rate of infection of grain mold (grade 7.0), compared to simple UA, AIC-2, and AIC-1 treatments (7.4, 7.6, and 8.0 grade, resp.). The HPLC quantification of differentially induced phenolic acids in treated sorghum grains substantiated this effect disclosing the higher accumulation of chlorogenic, vanillic, and salicylic acids in FCP. This might be due to defensive induction of these acids by the plants. Although mold control by examined chemicals were lesser than the standard Tilt (grade 5.9), they were found to be nontoxic to mammalian cells under cytotoxicity assay. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text