Characterization of a T-cell subset prevalent in immunoregulatory disorders in humans
| Autor: | Edwin B. Walker, Mahin M. Park |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Cytotoxicity Immunologic Sialoglycoproteins T-Lymphocytes ZAP70 Immunology Immunologic Deficiency Syndromes T lymphocyte Biology Lymphocyte Activation Natural killer T cell Autoimmune Diseases Interleukin 21 Humans Cytotoxic T cell IL-2 receptor Lymphocyte Culture Test Mixed Antigen-presenting cell CD8 |
| Zdroj: | Cellular Immunology. 111:265-272 |
| ISSN: | 0008-8749 |
| DOI: | 10.1016/0008-8749(88)90092-5 |
| Popis: | T cells from individuals with certain autoimmune diseases (rheumatoid arthritis, graft-versus-host disease, acquired immunodeficiency syndrome) express high levels of a cell surface sialoglycoprotein with a molecular weight of 140 kDa (gp140). Although a low frequency of gp140 + T cells was detected in the blood of normal individuals, upon stimulation with autologous EBV-transformed B cells (AMLR), the frequency of expression of gp140 was increased threefold. To further characterize gp140 + T cells, rosetting techniques with ox erythrocytes coated with monoclonal anti-gp140 antibody were used to isolate T-cell subsets for phenotypic, cell cycle, and functional analysis. The majority of gp140 + T cells expressed cytotoxic/suppressor (CD8 + ) phenotype in both normal and AMLR-activated states. Unstimulated gp140 + T cells had significantly greater nucleic acid content, as measured by acridine orange and flow cytometry, than gp140 − T cells. Surprisingly, the gp140 + T-cell subset had a less proliferative response in vitro to pokeweed or phytohemaglutinin mitogens. These results suggest that gp140 + T cells in normal individuals and in patients with autoimmune diseases may have been activated previously in vivo and that they are relatively resistant to reactivation in vitro . |
| Databáze: | OpenAIRE |
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