Real-time luminescence microspectroscopy monitoring of singlet oxygen in individual cells
| Autor: | Jan Hála, Jan Valenta, Roman Dědic, Marek Scholz, Thomas Breitenbach |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Luminescence
Porphyrins Noise (electronics) Mice chemistry.chemical_compound Optics Computer Systems Animals Photosensitizer Physical and Theoretical Chemistry Spectrograph Photosensitizing Agents Singlet Oxygen Singlet oxygen business.industry Detector 3T3 Cells Fibroblasts Photochemical Processes chemistry Microspectrophotometry Optoelectronics Single-Cell Analysis business Phosphorescence |
| Zdroj: | Scholz, M, Dědic, R, Valenta, J, Breitenbach, T & Hála, J 2014, ' Real-time luminescence microspectroscopy monitoring of singlet oxygen in individual cells ', Photochemical & Photobiological Sciences, vol. 13, no. 8, pp. 1203-1212 . https://doi.org/10.1039/c4pp00121d |
| ISSN: | 1474-9092 1474-905X |
| DOI: | 10.1039/c4pp00121d |
| Popis: | A new setup for direct microspectroscopic monitoring of singlet oxygen ((1)O2) has been developed in our laboratory using a novel near-infrared sensitive InGaAs 2D-array detector. An imaging spectrograph has been inserted in front of the 2D-array detector, which allows us to acquire spectral images where one dimension is spatial and the other is spectral. The work presents a detailed examination of sensitivity and noise characteristics of the setup and its ability to detect (1)O2. The (1)O2 phosphorescence-based images and near-infrared luminescence spectral images recorded from single TMPyP-containing fibroblast cells reflecting spectral changes during irradiation are demonstrated. The introduction of spectral images addresses the issue of a potential spectral overlap of (1)O2 phosphorescence with near-infrared-extended luminescence of the photosensitizer and provides a powerful tool for distinguishing and separating them, which can be applied to any photosensitizer manifesting near-infrared luminescence. |
| Databáze: | OpenAIRE |
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