Regulation of aromatase expression in the anterior amygdala of the developing mouse brain depends on ERβ and sex chromosome complement
| Autor: | María Julia Cambiasso, María Ángeles Arévalo, Lucas Ezequiel Cabrera Zapata, Carla D. Cisternas, Luis M. Garcia-Segura |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male AROMATASE Flutamide purl.org/becyt/ford/1 [https] chemistry.chemical_compound Mice 0302 clinical medicine Aromatase Cells Cultured Regulation of gene expression Neurons Multidisciplinary Sex Chromosomes biology Estradiol Gene Expression Regulation Developmental Dihydrotestosterone Amygdala Medicine Female GPER CIENCIAS NATURALES Y EXACTAS SEX CHROMOSOME COMPLEMENT medicine.drug Agonist medicine.medical_specialty medicine.drug_class Otras Ciencias Biológicas Science Article Ciencias Biológicas 03 medical and health sciences Internal medicine medicine Animals Estrogen Receptor beta purl.org/becyt/ford/1.6 [https] Estrogen receptor beta ESTROGEN RECEPTOR BETA PHTPP AMYGDALA 030104 developmental biology Endocrinology chemistry biology.protein 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Digital.CSIC. Repositorio Institucional del CSIC instname Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) |
| ISSN: | 2045-2322 |
| Popis: | During development sex differences in aromatase expression in limbic regions of mouse brain depend on sex chromosome factors. Genes on the sex chromosomes may affect the hormonal regulation of aromatase expression and this study was undertaken to explore that possibility. Male E15 anterior amygdala neuronal cultures expressed higher levels of aromatase (mRNA and protein) than female cultures. Furthermore, treatment with oestradiol (E2) or dihydrotestosterone (DHT) increased Cyp19a1 expression and aromatase protein levels only in female neuronal cultures. The effect of E2 on aromatase expression was not imitated by oestrogen receptor (ER) ¿ agonist PPT or the GPER agonist G1, but it was fully reproduced by DPN, a specific ligand of ERß. By contrast, the effect of DHT on aromatase expression was not blocked by the anti-androgen flutamide, but completely abrogated by the ERß antagonist PHTPP. Experiments using the four core genotype model showed a sex chromosome effect in ERß expression (XY¿>¿XX) and regulation by E2 or DHT (only XX respond) in amygdala neurons. In conclusion, sex chromosome complement governs the hormonal regulation of aromatase expression through activation of ERß in developing mouse brain. |
| Databáze: | OpenAIRE |
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