Supplementary Methods, Supplementary Figures 1 through 6, and Supplementary Table 1 from CIB2 Negatively Regulates Oncogenic Signaling in Ovarian Cancer via Sphingosine Kinase 1

Autor: Stuart M. Pitson, Melissa R. Pitman, Martin K. Oehler, Paul A.B. Moretti, Houng Taing, Briony L. Gliddon, Lorena T. Davies, Heidi A. Neubauer, Noor A. Lokman, Kate E. Jarman, Wenying Zhu
Rok vydání: 2023
Popis: Supplementary Methods. Supplementary Figure S1. Validation of the physiological interaction between CIB2 and SK1 using proximity ligation assays with different antibody combinations. Supplementary Figure S2. N-myristoylation of CIB2 does not affect its interaction with SK1. Supplementary Figure S3. Over-expression of CIB2 sensitises cells to TNFα-induced caspase 3/7 activation. Supplementary Figure S4. Stratification of CIB2 gene expression in ovarian cancer tissues. Supplementary Figure S5. Gene expression of SK1 in ovarian tissues. Supplementary Figure S6. CIB2 inhibits neoplastic growth and cell migration of OV90 ovarian cancer cells. Supplementary Table S1. Clinical characteristics of the ovarian cancer patient cohort from which ovirian cancer cells were derived.
Databáze: OpenAIRE