Contrast-enhanced microCT (EPIC-µCT) ex vivo applied to the mouse and human jaw joint
| Autor: | G A P Renders, A S Lin, G E J Langenbach, Vincent Everts, R E Guldberg, L Mulder, J.H. Koolstra |
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| Přispěvatelé: | Oral Cell Biology, Orale Celbiologie (ORM, ACTA) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Cartilage
Articular X-ray microtomography Contrast Media Mice Inbred Strains Osteoarthritis Knee Joint EPIC Mice Imaging Three-Dimensional stomatognathic system medicine Cadaver Image Processing Computer-Assisted Ioxaglic Acid Animals Humans Radiology Nuclear Medicine and imaging General Dentistry Mandibular Condyles Temporomandibular Joint business.industry Cartilage Mandibular Condyle Osteophyte General Medicine Anatomy X-Ray Microtomography Temporomandibular Joint Disorders medicine.disease Temporomandibular joint Mice Inbred C57BL Radiographic Image Enhancement stomatognathic diseases medicine.anatomical_structure Otorhinolaryngology Feasibility Studies Female business Ex vivo Biomedical engineering Research Article |
| Zdroj: | Renders, G A P, Mulder, L, Lin, A S, Langenbach, G E J, Koolstra, J H, Guldberg, R E & Everts, V 2014, ' Contrast-enhanced microCT (EPIC-µCT) ex vivo applied to the mouse and human jaw joint ', Dentomaxillofacial Radiology, vol. 43, no. 2, pp. 20130098 . https://doi.org/10.1259/dmfr.20130098 Dentomaxillofacial Radiology, 43(2). British Institute of Radiology |
| ISSN: | 0250-832X |
| Popis: | Objectives:The temporomandibular joint (TMJ) is susceptive to the development of osteoarthritis (OA). More detailed knowledge of its development is essential to improve our insight into TMJ-OA. It is imperative to have a standardized reliable three-dimensional (3D) imaging method that allows for detailed assessment of both bone and cartilage in healthy and diseased joints. We aimed to determine the applicability of a contrast-enhanced microCT (µCT) technique for ex vivo research of mouse and human TMJs.Methods:Equilibrium partitioning of an ionic contrast agent via µCT (EPIC-µCT) was previously applied for cartilage assessment in the knee joint. The method was ex vivo, applied to the mouse TMJ and adapted for the human TMJ.Results:EPIC-µCT (30-min immersion time) was applied to mouse mandibular condyles, and 3D imaging revealed an average cartilage thickness of 110 ± 16 µm. These measurements via EPIC-µCT were similar to the histomorphometric measures (113 ± 19 µm). For human healthy OA-affected TMJ samples, the protocol was adjusted to an immersion time of 1 h. 3D imaging revealed a significant thicker cartilage layer in joints with early signs of OA compared with healthy joints (414.2 ± 122.6 and 239.7 ± 50.5 µm, respectively). A subsequent significant thinner layer was found in human joints with late signs of OA (197.4 ± 159.7 µm).Conclusions:The EPIC-µCT technique is effective for the ex vivo assessment of 3D cartilage morphology in the mouse as well as human TMJ and allows bone-cartilage interaction research in TMJ-OA. |
| Databáze: | OpenAIRE |
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