Detection of the C protein gene among group B streptococci using PCR
| Autor: | A. J. H. Simpson, S. R. Heard, J. A. Mawn |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Base pair
Molecular Sequence Data Biology Polymerase Chain Reaction Sensitivity and Specificity Streptococcus agalactiae Pathology and Forensic Medicine law.invention Microbiology Nucleic acid thermodynamics Bacterial Proteins law Gene Polymerase chain reaction Southern blot Base Sequence Gene Amplification Nucleic acid sequence Nucleic Acid Hybridization General Medicine Molecular biology Blotting Southern Genes Bacterial Agarose gel electrophoresis Primer (molecular biology) Research Article |
| Zdroj: | Journal of Clinical Pathology. 46:633-636 |
| ISSN: | 0021-9746 |
| Popis: | AIM--To develop a polymerase chain reaction (PCR) for the specific detection of the C protein gene in strains of group B Streptococcus. METHODS--A single primer pair derived from the nucleotide sequence of the IgA binding beta antigen of the C protein complex permitted the specific amplification of a 592 base pair DNA fragment from the C protein gene. After 35 cycles of amplification this product could be detected by agarose gel electrophoresis. Southern blot hybridisation confirmed that this product was the C protein gene. RESULTS--PCR detected the C protein gene in 75 (63%) of 119 strains of group B streptococci analysed. The product was not detected in other Gram positive organisms, showing that this PCR assay was highly specific. The sensitivity of the assay was satisfactory to a dilution of 1 in 10,000 of extracted DNA. CONCLUSIONS--The C protein of group B streptococci is associated with neonatal sepsis. The specific detection of the C protein gene by PCR may help identify which strains are likely to be associated with infection by the organism. |
| Databáze: | OpenAIRE |
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