The 82F Late Puff Contains the L82 Gene, an Essential Member of a Novel Gene Family
| Autor: | Garza D, Stowers Rs, Steven Russell |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
DNA
Complementary Transgene Molecular Sequence Data Genes Insect Biology Homology (biology) chemistry.chemical_compound stomatognathic system Gene expression Animals Amino Acid Sequence RNA Messenger Cloning Molecular ecdysone Molecular Biology Gene In Situ Hybridization late puff Genetics Polytene chromosome Base Sequence Models Genetic Sequence Homology Amino Acid metamorphosis Metamorphosis Biological Gene Expression Regulation Developmental Cell Biology Phenotype genomic DNA chemistry Multigene Family Mutation Insect Proteins Drosophila Ecdysone Developmental Biology |
| Zdroj: | Developmental Biology. (1):116-130 |
| ISSN: | 0012-1606 |
| DOI: | 10.1006/dbio.1999.9358 |
| Popis: | Metamorphosis in Drosophila results from a hierarchy of ecdysone-induced gene expression initiated at the end of the third larval instar. A now classical model of this hierarchy was proposed based on observations of the activity of polytene chromosome “puffs” which distinguished “early” puffs as those directly induced by ecdysone and “late” puffs as those which become active as a secondary response to the hormone. We report here the isolation and characterization of the L82 gene corresponding to the extensively characterized late puff at 82F. L82 is a complex gene that spans at least 50 kb of genomic DNA, produces at least seven different nested mRNAs, and has homology to a novel gene family. In contrast to most previously characterized puff genes, the broad developmental expression pattern of L82 suggests that it is controlled by both ecdysone-dependent and ecdysone-independent regulatory mechanisms. L82 mutations were identified by transgene rescue of developmental delay and eclosion lethal phenotypes. |
| Databáze: | OpenAIRE |
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