Human Umbilical Cord Blood–Derived Endothelial Cells Reendothelialize Vein Grafts and Prevent Thrombosis
| Autor: | Neil J. Freedman, Lisheng Zhang, Melissa A. Brown, George A. Truskey, Lisa L. Satterwhite, Vrad W. Levering, Leigh Brian, Jiao-Hui Wu |
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| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Pathology Endothelium Umbilical cord Article Veins Mice Postoperative Complications Blood vessel prosthesis medicine Animals Humans Cells Cultured Neointimal hyperplasia Wound Healing business.industry Graft Occlusion Vascular Endothelial Cells Thrombosis Fetal Blood medicine.disease Blood Vessel Prosthesis Surgery Endothelial stem cell Venous thrombosis medicine.anatomical_structure Endothelium Vascular Cardiology and Cardiovascular Medicine Vein graft disease business |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology. 30:2150-2155 |
| ISSN: | 1524-4636 1079-5642 |
| DOI: | 10.1161/atvbaha.110.207076 |
| Popis: | Objective— To accelerate vein graft reendothelialization and reduce vein graft thrombosis by infusing human umbilical cord blood–derived endothelial cells (hCB-ECs) because loss of endothelium contributes to vein graft thrombosis and neointimal hyperplasia. Methods and Results— Under steady flow conditions in vitro, hCB-ECs adhered to smooth muscle cells 2.5 to 13 times more than ECs derived from peripheral blood or human aorta ( P 5 β 1 integrin heterodimers per cell ( P P scid /J mice. Two weeks postoperatively, vein grafts from hCB-EC–treated mice demonstrated approximately 55% reendothelialization and no luminal thrombosis. In contrast, vein grafts from sham-treated mice demonstrated luminal thrombosis in 75% of specimens ( P Conclusion— The hCB-ECs adhere to smooth muscle cells under flow conditions in vitro, accelerate vein graft reendothelialization in vivo, and prevent vein graft thrombosis. Thus, hCB-ECs offer novel therapeutic possibilities for vein graft disease. |
| Databáze: | OpenAIRE |
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