Expression of the avian gag-myc oncogene in Saccharomyces cerevisiae
| Autor: | P. Durrens, A. Fournier, Michel Aigle, L. Desfarges |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Transcription
Genetic viruses Genetic Vectors Molecular Sequence Data Saccharomyces cerevisiae Genes myc Gene Expression Biology Birds Transformation Genetic Plasmid Gene expression Genetics Animals Northern blot Cloning Molecular Gene Expression vector Base Sequence Oncogene Oncogenes General Medicine Blotting Northern biology.organism_classification Genes gag Precipitin Tests Molecular biology Heterologous expression Chromosomes Fungal Plasmids |
| Zdroj: | Current Genetics. 18:7-12 |
| ISSN: | 1432-0983 0172-8083 |
| DOI: | 10.1007/bf00321108 |
| Popis: | The gag-myc oncogenic sequence of the avian retrovirus MC29 was first inserted in a multicopy expression vector allowing its expression in Saccharomyces cerevisiae. The oncogene transcripts were detected in yeast by Northern blot hybridization and gag-myc proteins were revealed by immunoprecipitation. On solid medium, the average size of gag-myc transformant colonies was smaller than control. In liquid cultures, the gag-myc strains had a doubling time of 4.7 h compared with 3.1 h in the controls. In one of the recipient strains, and after an initial transient period of 5 days, the gag-myc transformants became physiologically indistinguishable from control. In another recipient strain, the slow-growth phenotype is permanent. Plasmid instability is increased in gag-myc transformants. When a single copy of the gag-myc gene was inserted in a yeast chromosome, no phenotype was observed, showing that slow growth is the consequence of plasmid loss. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink