Cannabinoid receptor Type 1 densities reflect social organization inMicrotus
| Autor: | Nicholas S. Lackey, Trenton C. Simmons, Brooke K. Dreyer, Sara M. Freeman, Devanand S. Manoli, Karen L. Bales |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Cannabinoid receptor Hippocampus Ligands Article Receptor Cannabinoid CB2 Radioligand Assay Sexual Behavior Animal 03 medical and health sciences Diencephalon 0302 clinical medicine Receptor Cannabinoid CB1 Species Specificity medicine Animals Microtus Cannabinoid Receptor Antagonists Brain Chemistry Pair Bond Sex Characteristics biology Arvicolinae Cerebrum General Neuroscience biology.organism_classification Prairie vole Thiazoles 030104 developmental biology medicine.anatomical_structure nervous system Organ Specificity Evolutionary biology Cerebellar cortex Female lipids (amino acids peptides and proteins) Vole Nerve Net Rimonabant Spleen 030217 neurology & neurosurgery |
| Zdroj: | J Comp Neurol |
| ISSN: | 1096-9861 0021-9967 |
| DOI: | 10.1002/cne.24996 |
| Popis: | Across many species, endocannabinoids play an important role in regulating social play, reward, and anxiety. These processes are mediated through at least two distinct cannabinoid receptors (CB), CB1 and CB2. CB1 expression is found in appreciable densities across regions of the brain that integrate memory with socio-spatial information; many of these regions have been directly linked to the neurobiology of pair bonding in monogamous species. Using receptor autoradiography, we provide the first distributional map of CB1 within the brains of closely related monogamous prairie voles and promiscuous meadow voles, and compare receptor densities across sexes and species in limbic regions. We observe CB1-specific signal using [3H] CP-55,940 and [3H] SR141716A, though the latter exhibited a lower signal to noise ratio. We confirmed the presence of CB2 in prairie vole spleen tissue using [3H] CP-55,940. However, we found no evidence of CB2 in the brain using either [3H] CP-55,940 or [3H] A-836,339. The overall distribution of putative CB1 in the brain was similar across vole species and followed the pattern of CB1 expression observed in other species-high intensity binding within the telencephalon, moderate binding within the diencephalon, and mild binding within the mesencephalon and metencephalon (aside from the cerebellar cortex). However, we found profound differences in CB1 densities across species, with prairie voles having higher CB1 binding in regions implicated in social attachment and spatial memory (e.g., periaqueductal gray, hippocampus). These findings suggest that CB1 densities, but not distribution, correlate with the social systems of vole species. |
| Databáze: | OpenAIRE |
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