Up-regulation of growth factor independence 1 in endotoxin tolerant macrophages with low secretion of TNF-α and IL-6
| Autor: | Sheng-Fu Dong, Hanssa Summah, Jieming Qu, Min-Fang Zhu, Jing Zhang, Shuang-Cheng Zhou, Hui-jun Zhang, Hong-Ni Jiang |
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| Rok vydání: | 2010 |
| Předmět: |
Lipopolysaccharides
Male Lipopolysaccharide medicine.medical_treatment Immunology Cell Line Mice chemistry.chemical_compound Downregulation and upregulation medicine Animals Secretion Interleukin 6 Pharmacology biology Interleukin-6 Tumor Necrosis Factor-alpha Macrophages Growth factor Molecular biology In vitro Up-Regulation DNA-Binding Proteins Mice Inbred C57BL Blot chemistry Cell culture biology.protein Transcription Factors |
| Zdroj: | Inflammation Research. 59:855-860 |
| ISSN: | 1420-908X 1023-3830 |
| Popis: | Endotoxin tolerance refers to a low response to lipopolysaccharide (LPS). We hypothesized that growth factor independence 1 (Gfi1) involves in the endotoxin tolerance in macrophages.Endotoxin tolerance was induced in the RAW264.7 cell line and thioglycolate-elicited murine peritoneal macrophages by incubation with 100 ng/ml LPS for 20 h. Macrophages without the pretreatment were set as control. Both endotoxin tolerant and control cells were then stimulated with 1,000 ng/ml LPS for indicated period of incubation. Gfi1 mRNA expression and protein production were investigated by real-time PCR and Western blotting, respectively. ELISA was performed to quantify the secretion of TNF-alpha and IL-6.Compared with non-endotoxin tolerant macrophages, endotoxin tolerant cells secreted a lower amount of TNF-alpha and IL-6. The mRNA expression of Gfi1 in endotoxin tolerant macrophages was higher than that of control in both RAW264.7 cells and thioglycolate-elicited murine peritoneal macrophages. The protein production was accordingly up-regulated in endotoxin tolerant RAW264.7 cells.In in vitro endotoxin tolerant macrophages, the expression of Gfi1 mRNA and protein were up-regulated after high dose LPS stimulation, accompanied with a blunted TNF-alpha and IL-6 secretion. Gfi1 might participate in the mechanism of endotoxin tolerance. |
| Databáze: | OpenAIRE |
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