Extracellular histones are the ligands for the uptake of exosomes and hydroxyapatite‐nanoparticles by tumor cellsviasyndecan‐4
| Autor: | Jeffrey S. Goodwin, Cierra Moye, Tanu Rana, Josiah Ochieng, Gladys N. Nangami, Amos M. Sakwe, Philip E. Lammers, Samuel E. Adunyah, Shalonda M. Ingram |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
alpha-2-HS-Glycoprotein Cell Biophysics Tumor cells Exosomes Ligands Biochemistry Article Syndecan 1 Histones 03 medical and health sciences Structural Biology Cell Line Tumor Neoplasms Genetics Extracellular medicine Humans Receptor Molecular Biology Incubation Microscopy Confocal biology Chemistry Cell Membrane Cell Biology Endocytosis Microvesicles Cell biology Durapatite 030104 developmental biology Histone medicine.anatomical_structure PC-3 Cells biology.protein Nanoparticles RNA Interference Syndecan-4 Extracellular Space |
| Zdroj: | FEBS Letters. 592:3274-3285 |
| ISSN: | 1873-3468 0014-5793 |
| DOI: | 10.1002/1873-3468.13236 |
| Popis: | The mechanisms by which exosomes (nano-vesicular messengers of cells) are taken up by recipient cells are poorly understood. We hypothesized that histones associated with these nano-particles are the ligands which facilitate their interaction with cell surface syndecan-4 (SDC4) to mediate their uptake. We show that the incubation with fetuin-A (exosome-associated proteins) and histones mediates the uptake of exosomes that are normally not endocytosed. Similarly, hydroxyapatite nanoparticles incubated with fetuin-A and histones (FNH) are internalized by tumor cells, while nanoparticles incubated with fetuin-A alone (FN) are not. The uptake of exosomes and FNH, both of which move to the perinuclear region of the cell, is attenuated in SDC4-knockdown cells. Data show that FNH can compete with exosomes for uptake and that both use SDC4 as uptake receptors. |
| Databáze: | OpenAIRE |
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