Physiological and molecular analysis of a mecA-negative Staphylococcus aureus clinical strain that expresses heterogeneous methicillin resistance
| Autor: | Kyoko Kuwahara-Arai, Tadashi Baba, Reiko Yoshida, Judith F. Richardson, Keiichi Hiramatsu, Longzhu Cui |
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| Rok vydání: | 2003 |
| Předmět: |
Microbiology (medical)
Staphylococcus aureus Micrococcaceae Meticillin Penicillin binding proteins Blotting Western Microbial Sensitivity Tests Penicillins Muramoylpentapeptide Carboxypeptidase medicine.disease_cause beta-Lactamases Microbiology Acetylglucosamine Cell wall Methicillin Bacterial Proteins Cell Wall medicine Penicillin-Binding Proteins Pharmacology (medical) In Situ Hybridization Antibacterial agent Pharmacology biology Strain (chemistry) Reverse Transcriptase Polymerase Chain Reaction biochemical phenomena metabolism and nutrition Penicillinase Staphylococcal Infections bacterial infections and mycoses biology.organism_classification Anti-Bacterial Agents Microscopy Electron Infectious Diseases Hexosyltransferases Peptidyl Transferases Methicillin Resistance Autolysis Carrier Proteins Bacteria medicine.drug Plasmids |
| Zdroj: | The Journal of antimicrobial chemotherapy. 51(2) |
| ISSN: | 0305-7453 |
| Popis: | Staphylococcus aureus clinical isolate 61/5896 exhibited methicillin resistance (MIC 64 mg/L), but lacked mecA, which encodes penicillin-binding protein 2'. The strain was isolated in England in 1961, and exhibited unstable heterogeneous methicillin resistance. When cultivated in drug-free medium, the methicillin resistance of 61/5896 increased after three daily passages, then decreased and was completely lost after 12 days' passage. Electron microscopy revealed that strain 61/5896 had a thicker and rougher cell wall than its methicillin-susceptible derivatives. It produced about three times more penicillin-binding protein 2 (PBP2) than methicillin-susceptible derivatives. The strain was characteristically a non-producer of autolytic enzyme, though the phenotype, which was lost easily, was not directly correlated with methicillin resistance. |
| Databáze: | OpenAIRE |
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