Pitfalls in interpretation of CT-values of RT-PCR in children with acute respiratory tract infections

Autor: Tjeerd van der Ploeg, Nico G. Hartwig, Leo C. Smeets, Ronald de Groot, Jérôme O. Wishaupt, Florens G. A. Versteegh
Přispěvatelé: Pediatrics
Rok vydání: 2017
Předmět:
0301 basic medicine
FLU
influenza virus
viruses
030106 microbiology
lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4]
Real-Time Polymerase Chain Reaction
medicine.disease_cause
Severity of Illness Index
Article
03 medical and health sciences
0302 clinical medicine
All institutes and research themes of the Radboud University Medical Center
Human metapneumovirus
HBoV
human bocavirus
Respiratory infection
Virology
medicine
Humans
Viral load
Clinical significance
Prospective Studies
030212 general & internal medicine
LOS
length of hospital stay
Child
HMPV
human metapneumovirus
Disease severity
Respiratory Tract Infections
Acute respiratory tract infection
RT-PCR
real-time reverse transcription-polymerase chain reaction test
biology
Respiratory tract infections
Reverse Transcriptase Polymerase Chain Reaction
Human bocavirus
biology.organism_classification
PIV
parainfluenza virus
RV
rhinovirus
CT
cycle threshold value
Infectious Diseases
Virus Diseases
HCoV
human coronavirus
Child
Preschool
Immunology
HAdV
human adenovirus
RSV
respiratory syncytial virus
Rhinovirus
Cycle threshold value
ARI
acute respiratory tract infection
Zdroj: Journal of Clinical Virology, 90, pp. 1-6
Journal of Clinical Virology, 90, 1-6. Elsevier
Journal of Clinical Virology, 90, 1-6
Journal of Clinical Virology
ISSN: 1386-6532
Popis: Background The relation between viral load and disease severity in childhood acute respiratory tract infections (ARI) is not fully understood. Objectives To assess the clinical relevance of the relation between viral load, determined by cycle threshold (CT) value of real-time reverse transcription-polymerase chain reaction assays and disease severity in children with single- and multiple viral ARI. Study design 582 children with ARI were prospectively followed and tested for 15 viruses. Correlations were calculated between CT values and clinical parameters. Results In single viral ARI, statistically significant correlations were found between viral loads of Respiratory Syncytial Virus (RSV) and hospitalization and between viral loads of Human Coronavirus (HCoV) and a disease severity score. In multiple-viral ARI, statistically significant correlations between viral load and clinical parameters were found. In RSV-Rhinovirus (RV) multiple infections, a low viral load of RV was correlated with a high length of hospital stay and a high duration of extra oxygen use. The mean CT value for RV, HCoV and Parainfluenza virus was significantly lower in single- versus multiple infections. Conclusion Although correlations between CT values and clinical parameters in patients with single and multiple viral infection were found, the clinical importance of these findings is limited because individual differences in host-, viral and laboratory factors complicate the interpretation of statistically significant findings. In multiple infections, viral load cannot be used to differentiate between disease causing virus and innocent bystanders.
Databáze: OpenAIRE
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