Induction of donor-specific tolerance in rat hind-limb allografts under antilymphocyte serum and cyclosporine A protocol
| Autor: | D. Iżycki, Rita Prajapati, Maria Siemionow, Kagan Ozer, Ramadan Oke |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Graft Rejection
Male medicine.medical_specialty T-Lymphocytes medicine.medical_treatment Urology Immune tolerance Rats Sprague-Dawley Antigen Immune Tolerance medicine Animals Transplantation Homologous Orthopedics and Sports Medicine Antilymphocyte Serum Transplantation Chimera Chemotherapy business.industry Graft Survival Immunosuppression Flow Cytometry Mixed lymphocyte reaction Ciclosporin Hindlimb Rats Transplantation Immunology Cyclosporine Surgery Immunocompetence business Immunosuppressive Agents medicine.drug |
| Zdroj: | The Journal of Hand Surgery. 27:1095-1103 |
| ISSN: | 0363-5023 |
| DOI: | 10.1053/jhsu.2002.36524 |
| Popis: | Composite tissue allograft (CTA) transplantation became a clinical reality despite major side effects associated with the administration of chronic immunosuppression. Development of new treatment modalities eliminating life-long immunosuppression is essential for the future of CTA transplantation. In this study, combined use of cyclosporine A (CsA) and antilymphocyte serum (ALS) was tested for the potential to induce tolerance in the rat hind-limb allograft recipients across a major histocompatibility (MHC) barrier (Lewis-Brown-Norway [LBN, RT1 l+n ] to Lewis [LEW, RT1 l ] rats). Thirty transplantations were performed in 5 experimental groups. Animals received CsA and ALS 12 hours before surgery for 21 days thereafter. Although the allograft controls rejected their limbs at day 7 combined treatment of CsA and ALS resulted in indefinite survival (over 420 d) in all allograft recipients. Long-term survivors showed 35% to 42% of donor-specific chimerism in the peripheral blood. Clinical tolerance was confirmed by acceptance of the donor-specific skin grafts and immunocompetence was confirmed by rejection of the third-party grafts. Mixed lymphocyte reaction revealed suppressed response against donor-type antigens and increased response to third-party antigens. Donor-specific tolerance across MHC barrier was induced in CTA allografts under 21 days protocol of ALS/CsA. (J Hand Surg 2002;27A:1095–1103. Copyright © 2002 by the American Society for Surgery of the Hand.) |
| Databáze: | OpenAIRE |
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