Characterization of transforming growth factor-α receptors in the avian ovary: alterations in ligand binding to granulosa cells during follicular maturation
| Autor: | M. J. Peddie, I Woolveridge, O. M. Onagbesan |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
TGF alpha Endocrinology Diabetes and Metabolism Granulosa cell Basic fibroblast growth factor Alpha (ethology) Biology Binding Competitive chemistry.chemical_compound Endocrinology Ovarian Follicle Epidermal growth factor Internal medicine medicine Animals Humans Receptor Cells Cultured Granulosa Cells Epidermal Growth Factor Ovary Luteinizing Hormone ErbB Receptors chemistry Female Folliculogenesis Follicle Stimulating Hormone Chickens Protein Binding Transforming growth factor |
| Zdroj: | Journal of Endocrinology. 149:171-179 |
| ISSN: | 1479-6805 0022-0795 |
| DOI: | 10.1677/joe.0.1490171 |
| Popis: | The presence of epidermal growth factor receptors (EGF-R) and the ligands epidermal growth factor/transforming growth factor-α (EGF/TGFα) have been reported in mammalian ovaries where they are implicated in folliculogenesis and steroidogenesis. Evidence is presented to show that authentic EGF/TGFα receptors are expressed by the avian granulosa cells. The TGFα receptors (TGFα-R) from chicken granulosa cells were characterized by specific binding of 125I-human TGFα. In this study, competition with human EGF, human TGFα, human IGF-I, human basic fibroblast growth factor (bFGF) and insulin for 125I-human TGFα binding demonstrated that the avian granulosa cell TGFα-R binds human EGF with 300-fold lower affinity than human TGFα. IGF-I, bFGF and insulin did not displace bound 125I-TGFα. Scatchard analysis showed that a single class of high-affinity binding sites is present on the granulosa cells (Kd 0·23 ± 0·009 nm). However, the number of binding sites altered during follicular maturation with a significant decline in the most mature follicle. These results go some way to explaining the basis for the changing sensitivity of avian granulosa cells to EGF/TGFα stimulation as they mature. In addition, the gonadotrophins, LH and FSH, increased the number of receptors in cultured granulosa cells and may therefore partially influence folliculogenesis and steroidogenesis through this route. Journal of Endocrinology (1996) 149, 171–179 |
| Databáze: | OpenAIRE |
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