Biological properties of ulvan, a new source of green seaweed sulfated polysaccharides, on cultured normal and cancerous colonic epithelial cells
| Autor: | Christine Cherbut, Marc Lahaye, Bertrand Kaeffer, Hervé M. Blottière, Claudine Benard |
|---|---|
| Přispěvatelé: | Unité de recherche Fonctions Digestives et de Nutrition Humaines (UFDNH), Institut National de la Recherche Agronomique (INRA), Laboratoire de biochimie et technologie des glucides |
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Dietary Fiber
[SDV]Life Sciences [q-bio] sulfated oligosaccharide Pharmaceutical Science Uronic acid Biology Polysaccharide 01 natural sciences Analytical Chemistry Glycosaminoglycan Colony-Forming Units Assay 03 medical and health sciences chemistry.chemical_compound Sulfation ulvan Polysaccharides Chlorophyceae Drug Discovery Cell Adhesion Tumor Cells Cultured Animals Humans Intestinal Mucosa Cytotoxicity 030304 developmental biology Pharmacology chemistry.chemical_classification 0303 health sciences Molecular mass colon 010405 organic chemistry Plant Extracts Organic Chemistry Biological activity Epithelial Cells Sulfuric Acids Seaweed In vitro 0104 chemical sciences Rats Complementary and alternative medicine chemistry Biochemistry Colonic Neoplasms Molecular Medicine epithelium Cell Division |
| Zdroj: | Planta Medica Planta Medica, Georg Thieme Verlag, 1999, 65, pp.527-531. ⟨10.1055/s-1999-14009⟩ ResearcherID |
| ISSN: | 0032-0943 1439-0221 |
| DOI: | 10.1055/s-1999-14009⟩ |
| Popis: | International audience; Ulvans (from Ulva lactuca) constitute a dietary fiber structurally similar to the mammalian glycosaminoglycans but with unexplored biological or cytotoxic activities. From native low-viscosity preparations containing 33.5 molar % and 18.4 molar % of sulfate residues and uronic acid residues, respectively, we derived desulfated, reduced and desulfated-reduced polysaccharides with respectively 5.2, 2.9, and 4.5-4.9 molar % of sulfate residues and uronic acid residues. The effects of these preparations were examined on the adhesion, proliferation and differentiation of normal or tumoral colonic epithelial cells cultured in conventional (0.3-0.8 x 10(6) cells/ml) or rotating bioreactor (3-8 x 10(6) cells/ml) culture conditions. In conventional culture conditions, ulvan modified the adhesion phase and the proliferation of normal colonic cells and undifferentiated HT-29 cells according to their molecular weights and to the relative molar proportion of sulfate residues. From the native polysaccharides, we have screened sulfated ulvans (MW < 5,000) which inhibited the Caco-2 cell proliferation/differentiation program by inducing a low cell reactivity to Ulex europeaus-1 lectins in defined (p < 0.001) or serum-supplemented media (p < 0.01) but were inactive on normal colonocytes. In conclusion, this dietary fiber could be a source of oligosaccharides with a bioactivity, a cytotoxicity or a cytostaticity targeted to normal or cancerous epithelial cells. |
| Databáze: | OpenAIRE |
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