3D-Printed Gelatin Methacryloyl-Based Scaffolds with Potential Application in Tissue Engineering
| Autor: | Jana Ghitman, Raluca Ianchis, Cristina Elena Stavarache, Horia Iovu, Andrada Serafim, Maria-Minodora Marin, Rebeca Leu Alexa |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
food.ingredient
Polymers and Plastics 02 engineering and technology 010402 general chemistry 01 natural sciences Gelatin Article Contact angle gelatin lcsh:QD241-441 food Tissue engineering lcsh:Organic chemistry medicine hydrogels GelMA chemistry.chemical_classification Chemistry General Chemistry Polymer 3D printing 021001 nanoscience & nanotechnology 0104 chemical sciences Photopolymer Chemical engineering Self-healing hydrogels photopolymerization Swelling medicine.symptom 0210 nano-technology Photoinitiator biomaterials |
| Zdroj: | Polymers, Vol 13, Iss 727, p 727 (2021) Polymers Volume 13 Issue 5 |
| ISSN: | 2073-4360 |
| Popis: | The development of materials for 3D printing adapted for tissue engineering represents one of the main concerns nowadays. Our aim was to obtain suitable 3D-printed scaffolds based on methacrylated gelatin (GelMA). In this respect, three degrees of GelMA methacrylation, three different concentrations of GelMA (10%, 20%, and 30%), and also two concentrations of photoinitiator (I-2959) (0.5% and 1%) were explored to develop proper GelMA hydrogel ink formulations to be used in the 3D printing process. Afterward, all these GelMA hydrogel-based inks/3D-printed scaffolds were characterized structurally, mechanically, and morphologically. The presence of methacryloyl groups bounded to the surface of GelMA was confirmed by FTIR and 1H-NMR analyses. The methacrylation degree influenced the value of the isoelectric point that decreased with the GelMA methacrylation degree. A greater concentration of photoinitiator influenced the hydrophilicity of the polymer as proved using contact angle and swelling studies because of the new bonds resulting after the photocrosslinking stage. According to the mechanical tests, better mechanical properties were obtained in the presence of the 1% initiator. Circular dichroism analyses demonstrated that the secondary structure of gelatin remained unaffected during the methacrylation process, thus being suitable for biological applications. |
| Databáze: | OpenAIRE |
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