Correlation of specific virus-astrocyte interactions and cytopathic effects induced by ts1, a neurovirulent mutant of Moloney murine leukemia virus
| ISSN: | 1098-5514 0022-538X |
|---|---|
| DOI: | 10.1128/jvi.67.3.1137-1147.1993 |
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc7c4ed209a87a8ff4291277b53b4c8e https://doi.org/10.1128/jvi.67.3.1137-1147.1993 |
| Rights: | OPEN |
| Přírůstkové číslo: | edsair.doi.dedup.....bc7c4ed209a87a8ff4291277b53b4c8e |
| Autor: | Kunal Saha, Ying-Chuan Lin, Paul K.Y. Wong, B. R. Brooks, E. Shikova |
| Rok vydání: | 1993 |
| Předmět: |
Immunology
Golgi Apparatus Mice Inbred Strains Endoplasmic Reticulum Microbiology Virus Mice symbols.namesake Cytopathogenic Effect Viral Viral Envelope Proteins Virology Moloney Murine Leukemia Virus TB Murine leukemia virus medicine Animals Diencephalon Protein Precursors Cells Cultured Cytopathic effect Virulence biology Endoplasmic reticulum Golgi apparatus biology.organism_classification Cell Compartmentation Cell killing medicine.anatomical_structure Astrocytes Insect Science Mutation symbols Endothelium Vascular Moloney murine leukemia virus Protein Processing Post-Translational Research Article Astrocyte |
| Zdroj: | Journal of Virology. 67:1137-1147 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.67.3.1137-1147.1993 |
| Popis: | ts1 is a highly neuropathogenic and lymphocytopathic mutant of Moloney murine leukemia virus TB (MoMuLV-TB). We previously reported that the primary neuropathogenic determinant of ts1 maps to a single amino acid substitution, Val-25-->Ile, in precursor envelope protein gPr80env. This Val-25-->Ile substitution apparently renders gPr80env inefficient for transport from the endoplasmic reticulum to the Golgi apparatus. These findings suggest that the cytopathic effect of ts1 in neural cells might be due to the accumulation of gPr80env in the endoplasmic reticulum. Since endothelial and glial cells are targets of ts1 infection in the central nervous system, we established primary endothelial and astrocyte cultures to investigate the mechanism of cell killing caused by ts1. A continuous cell line, TB, was used as a control. Our results showed that both ts1 and MoMuLV-TB replicated and induced a cytopathic effect in astrocyte cultures, albeit to different degrees; ts1 appeared to be more lethal than MoMuLV-TB. On the other hand, ts1 and MoMuLV-TB infections of endothelial or TB cells were not cytopathic. The cytopathic effect in infected astrocytes correlated with the inefficiency of gPr80env transport and the intracellular accumulation of gPr80env as well as aberrant virus particles. |
| Databáze: | OpenAIRE |
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