Correlation of Acinetobacter baumannii K144 and K86 capsular polysaccharide structures with genes at the K locus reveals the involvement of a novel multifunctional rhamnosyltransferase for structural synthesis
| Autor: | Andrei S. Dmitrenok, Anastasiya A. Kasimova, Johanna J. Kenyon, Anna M. Shpirt, Alexander O. Chizov, Anastasiya N. Sviridova, Andrei A. Shelenkov, Alexander S. Shashkov, Anastasiya V. Popova, Yuriy A. Knirel, Yuliya V. Mikhaylova, Mikhail M. Shneider, Andrei V. Perepelov |
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| Rok vydání: | 2021 |
| Předmět: |
Acinetobacter baumannii
Magnetic Resonance Spectroscopy Genetic Linkage Rhamnose Stereochemistry Locus (genetics) Biochemistry 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Structural Biology Gene cluster Trisaccharide Molecular Biology Gene 030304 developmental biology chemistry.chemical_classification 0303 health sciences Whole Genome Sequencing biology 030306 microbiology Polysaccharides Bacterial Glycosyltransferase Gene Glycosyltransferases Glycosidic bond General Medicine biology.organism_classification chemistry Multigene Family |
| Zdroj: | International Journal of Biological Macromolecules. 193:1294-1300 |
| ISSN: | 0141-8130 |
| Popis: | Whole genome sequence from Acinetobacter baumannii isolate Ab-46-1632 reveals a novel KL144 capsular polysaccharide (CPS) biosynthesis gene cluster, which carries genes for d -glucuronic acid ( D -GlcA) and l -rhamnose ( l -Rha) synthesis. The CPS was extracted from Ab-46-1632 and studied by 1H and 13C NMR spectroscopy, including a two-dimensional 1H,13C HMBC experiment and Smith degradation. The CPS was found to have a hexasaccharide repeat unit composed of four l -Rhap residues and one residue each of d -GlcpA and N-acetyl- d -glucosamine ( D -GlcpNAc) consistent with sugar synthesis genes present in KL144. The K144 CPS structure was established and found to be related to those of A. baumannii K55, K74, K85, and K86. A comparison of the corresponding gene clusters to KL144 revealed a number of shared glycosyltransferase genes correlating to shared glycosidic linkages in the structures. One from the enzymes, encoded by only KL144 and KL86, is proposed to be a novel multifunctional rhamnosyltransfaerase likely responsible for synthesis of a shared α- l -Rhap-(1 → 2)-α-L-Rhap-(1 → 3)-L-Rhap trisaccharide fragment in the K144 and K86 structures. |
| Databáze: | OpenAIRE |
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