The Complete Genome Sequence of Severe Acute Respiratory Syndrome Coronavirus Strain HKU-39849 (HK-39)
| Autor: | F.Y. Zeng, Kwok-Hung Chan, K.Y.C. Chow, Joseph S. M. Peiris, K.H. Hui, Chung-Chau Hon, Kwok-Yung Yuen, J.D. Chen, Yi Guan, C.Y. Wang, Frederick C. Leung, Che Man Chan, Bo-Jian Zheng, Jun Li, P.Y. Wang, Leo L.M. Poon, V.Y.Y. Li, M.N. Chan |
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| Rok vydání: | 2003 |
| Předmět: |
0301 basic medicine
DNA Complementary Transcription Genetic viruses Molecular Sequence Data Genome Viral Severe Acute Respiratory Syndrome medicine.disease_cause General Biochemistry Genetics and Molecular Biology Conserved sequence Open Reading Frames Viral Proteins 03 medical and health sciences 0302 clinical medicine medicine Coronaviridae Amino Acid Sequence ORFS 3' Untranslated Regions Conserved Sequence Phylogeny Coronavirus Sequence (medicine) Genetics Base Sequence Sequence Homology Amino Acid biology Nucleic acid sequence Sequence Analysis DNA Nucleic acid amplification technique biology.organism_classification 030104 developmental biology Severe acute respiratory syndrome-related coronavirus 030220 oncology & carcinogenesis Novel virus RNA Viral 5' Untranslated Regions Nucleic Acid Amplification Techniques |
| Zdroj: | Experimental Biology and Medicine. 228:866-873 |
| ISSN: | 1535-3699 1535-3702 |
| Popis: | The complete genomic nucleotide sequence (29.7kb) of a Hong Kong severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) strain HK-39 is determined. Phylogenetic analysis of the genomic sequence reveals it to be a distinct member of the Coronaviridae family. 5′ RACE assay confirms the presence of at least six subgenomic transcripts all containing the predicted intergenic sequences. Five open reading frames (ORFs), namely ORF1a, 1b, S, M, and N, are found to be homologues to other CoV members, and three more unknown ORFs (X1, X2, and X3) are unparalleled in all other known CoV species. Optimal alignment and computer analysis of the homologous ORFs has predicted the characteristic structural and functional domains on the putative genes. The overall nucleotides conservation of the homologous ORFs is low ( |
| Databáze: | OpenAIRE |
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