In Vitro Cell Motility as a Potential Mesenchymal Stem Cell Marker for Multipotency
Autor: | Jivko Stoyanov, Martin Baur, Marco Gruber, Benjamin Gantenbein, Tobias Pötzel, Armin Gemperli, Alessandro Bertolo |
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Rok vydání: | 2014 |
Předmět: |
Cellular differentiation
Cell Population Motility In Vitro Techniques Biology Time-Lapse Imaging 03 medical and health sciences 0302 clinical medicine Cell Movement Tissue Engineering and Regenerative Medicine medicine Humans education Cells Cultured Cellular Senescence 030304 developmental biology 0303 health sciences education.field_of_study Multipotent Stem Cells Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Cell Biology General Medicine Anatomy Flow Cytometry Cell biology medicine.anatomical_structure Multipotent Stem Cell Bone marrow Cell aging Biomarkers 030217 neurology & neurosurgery Developmental Biology |
Zdroj: | Stem Cells Transl Med |
ISSN: | 2157-6580 2157-6564 |
Popis: | Mesenchymal stem cells (MSCs) are expected to have a fundamental role in future cell-based therapies because of their high proliferative ability, multilineage potential, and immunomodulatory properties. Autologous transplantations have the “elephant in the room” problem of wide donor variability, reflected by variability in MSC quality and characteristics, leading to uncertain outcomes in the use of these cells. We propose life imaging as a tool to characterize populations of human MSCs. Bone marrow MSCs from various donors and in vitro passages were evaluated for their in vitro motility, and the distances were correlated to the adipogenic, chondrogenic, and osteogenic differentiation potentials and the levels of senescence and cell size. Using life-image measuring of track lengths of 70 cells per population for a period of 24 hours, we observed that slow-moving cells had the higher proportion of senescent cells compared with fast ones. Larger cells moved less than smaller ones, and spindle-shaped cells had an average speed. Both fast cells and slow cells were characterized by a low differentiation potential, and average-moving cells were more effective in undergoing all three lineage differentiations. Furthermore, heterogeneity in single cell motility within a population correlated with the average-moving cells, and fast- and slow-moving cells tended toward homogeneity (i.e., a monotonous moving pattern). In conclusion, in vitro cell motility might be a useful tool to quickly characterize and distinguish the MSC population's differentiation potential before additional use. |
Databáze: | OpenAIRE |
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