Metabolic engineering for high yield synthesis of astaxanthin in Xanthophyllomyces dendrorhous
| Autor: | Jorge Verdín, Alejandro Torres-Haro, Manuel R. Kirchmayr, Melchor Arellano-Plaza |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Biological pigments
Mutant Bioengineering Review Xanthophylls Microbiology Applied Microbiology and Biotechnology Metabolic engineering chemistry.chemical_compound Astaxanthin Omics-based engineering Carbon flow Food science Carotenoid Biological pigment chemistry.chemical_classification Chemistry Basidiomycota Carotenoids QR1-502 Xanthophyllomyces dendrorhous Metabolic Engineering Yield (chemistry) Biotechnology |
| Zdroj: | Microbial Cell Factories Microbial Cell Factories, Vol 20, Iss 1, Pp 1-17 (2021) |
| ISSN: | 1475-2859 |
| Popis: | Astaxanthin is a carotenoid with a number of assets useful for the food, cosmetic and pharmaceutical industries. Nowadays, it is mainly produced by chemical synthesis. However, the process leads to an enantiomeric mixture where the biologically assimilable forms (3R, 3′R or 3S, 3′S) are a minority. Microbial production of (3R, 3′R) astaxanthin by Xanthophyllomyces dendrorhous is an appealing alternative due to its fast growth rate and easy large-scale production. In order to increase X. dendrorhous astaxanthin yields, random mutant strains able to produce from 6 to 10 mg/g dry mass have been generated; nevertheless, they often are unstable. On the other hand, site-directed mutant strains have also been obtained, but they increase only the yield of non-astaxanthin carotenoids. In this review, we insightfully analyze the metabolic carbon flow converging in astaxanthin biosynthesis and, by integrating the biological features of X. dendrorhous with available metabolic, genomic, transcriptomic, and proteomic data, as well as the knowledge gained with random and site-directed mutants that lead to increased carotenoids yield, we propose new metabolic engineering targets to increase astaxanthin biosynthesis. |
| Databáze: | OpenAIRE |
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