Performance of Two Novel Chromogenic Media for the Identification of Multidrug-Resistant Candida auris Compared with Other Commercially Available Formulations
| Autor: | Ferry Hagen, Mauricio Carbia, Chendo Dieleman, Ratna Mohd Tap, Auke W. de Jong |
|---|---|
| Přispěvatelé: | Westerdijk Fungal Biodiversity Institute, Westerdijk Fungal Biodiversity Institute - Medical Mycology |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
food.ingredient biology Candida glabrata Candida pseudohaemulonii Chromogenic Mycology biology.organism_classification Microbiology Culture Media Multiple drug resistance food Candida auris Chromogenic Compounds Species Specificity Saccharomycetales Agar Chromagar candida Humans Candida dubliniensis Candida |
| Zdroj: | Journal of Clinical Microbiology, 59(4). American Society for Microbiology J Clin Microbiol |
| ISSN: | 0095-1137 |
| Popis: | Non-albicans Candida species are emerging in the nosocomial environment, with the multidrug-resistant (MDR) species Candida auris being the most notorious example. Consequently, rapid and accurate species identification has become essential. The objective of this study was to evaluate five commercially available chromogenic media for the presumptive identification of C. auris. Two novel chromogenic formulations, CHROMagar Candida Plus (CHROMagar) and HiCrome C. auris MDR selective agar (HiMedia), and three reference media, CandiSelect (Bio-Rad), CHROMagar Candida (CHROMagar), and Chromatic Candida (Liofilchem), were inoculated with a collection of 9 genetically diverse C. auris strains and 35 strains from closely related comparator species. After 48 h of incubation, the media were evaluated for their ability to detect and identify C. auris. All media had the same limitations in the differentiation of the more common species Candida dubliniensis and Candida glabrata. Only on CHROMagar Candida Plus did C. auris colonies develop a species-specific coloration. Nevertheless, the closely related pathogenic species Candida pseudohaemulonii and Candida vulturna developed a similar appearance as C. auris on this medium. CHROMagar Candida Plus was shown to be superior in the detection and identification of C. auris, with 100% inclusivity for C. auris compared to 0% and 33% for the reference media and HiCrome C. auris MDR selective agar, respectively. Although C. vulturna and C. pseudohaemulonii can cause false positives, CHROMagar Candida Plus was shown to be a valuable addition to the plethora of mostly molecular methods for C. auris detection and identification. |
| Databáze: | OpenAIRE |
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