The protective effects of Cichorium glandulosum seed and cynarin against cyclophosphamide and its metabolite acrolein-induced hepatotoxicity in vivo and in vitro
| Autor: | Gao Zhou, Jing Tong, Youwei Wang, Bingxin Ma, Qigui Mo, Lan-Lan Ge |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
NF-E2-Related Factor 2 Metabolite Antineoplastic Agents Asteraceae Pharmacology Protective Agents Mice 03 medical and health sciences chemistry.chemical_compound In vivo medicine Animals Humans Acrolein Cytotoxicity Cyclophosphamide Glutathione Transferase chemistry.chemical_classification Liver injury Mice Inbred BALB C Reactive oxygen species Plant Extracts General Medicine Glutathione medicine.disease Cytosol 030104 developmental biology chemistry Cinnamates Seeds Female Chemical and Drug Induced Liver Injury Reactive Oxygen Species Food Science |
| Zdroj: | Food & Function. 8:209-219 |
| ISSN: | 2042-650X 2042-6496 |
| Popis: | Cyclophosphamide (CP) is a widely utilized chemotherapy drug. CP and its metabolite, acrolein, could induce hepatotoxicity. In this study, Cichorium glandulosum seed (CGS) effectively mitigated CP-induced hepatotoxicity in mice. Protection of cynarin, the major compound of CGS, against acrolein cytotoxicity in HepG2 cells was studied. Pretreatment with cynarin could improve cell survival against acrolein cytotoxicity. Cynarin restored the balance of glutathione (GSH) and reactive oxygen species (ROS), and inhibited mitochondrial depolarization. The kinetics of Nrf2 expression in cytosolic and nuclear fractions were observed after acrolein exposure. Intracellular Nrf2 expression was triggered within 6 h of exposure but did not translocate to the nucleus. Cynarin pretreatment ameliorated the expression and activity of GSH S-transferase and triggered Nrf2 nuclear translocation. In conclusion, treatment with CGS and cynarin protects liver injury against CP and acrolein hepatotoxicity via improvement of GSH activity and activation of the Nrf2 pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|