Viral expression and leukocyte adhesion after in vitro infection of goat mammary gland cells with caprine arthritis-encephalitis virus
| Autor: | Jean-François Mornex, I. Dreesen, C. Le Jan, P. Renson, N. Milhau, François Guiguen, Timothy Greenland, Claire Bellaton |
|---|---|
| Přispěvatelé: | Rétrovirus et Pathologie Comparée (RPC), Institut National de la Recherche Agronomique (INRA)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL), BioSciences Lyon-Gerland (BLG), École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-École Pratique des Hautes Études (EPHE), École Pratique des Hautes Études (EPHE), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL) |
| Rok vydání: | 2005 |
| Předmět: |
Cell type
Arthritis-Encephalitis Virus Caprine [SDV]Life Sciences [q-bio] EPITHELIUM Immunology Cell Mammary gland Polymerase Chain Reaction Flow cytometry 03 medical and health sciences Mammary Glands Animal Immune system Proviruses Antigen Cell Adhesion Leukocytes medicine Animals MYOEPITHELIUM Caprine arthritis encephalitis virus Cells Cultured 030304 developmental biology 0303 health sciences General Veterinary biology medicine.diagnostic_test Goats 0402 animal and dairy science Myoepithelial cell MAMMARY GLAND 04 agricultural and veterinary sciences biology.organism_classification 040201 dairy & animal science Virology Molecular biology LENTIVIRUS 3. Good health medicine.anatomical_structure ENDOTHELIUM FLOW CYTOMETRY Female |
| Zdroj: | Veterinary Immunology and Immunopathology Veterinary Immunology and Immunopathology, Elsevier, 2005, 103 (1-2), pp.93-99. ⟨10.1016/j.vetimm.2004.08.016⟩ Veterinary Immunology and Immunopathology, 2005, 103 (1-2), pp.93-99. ⟨10.1016/j.vetimm.2004.08.016⟩ |
| ISSN: | 0165-2427 |
| DOI: | 10.1016/j.vetimm.2004.08.016 |
| Popis: | International audience; A characteristic lesion in goats infected by the lentivirus CAEV is mastitis with lymphoid hyperplasia. In order to investigate the mechanism of lesion formation, cultures highly enriched in microvascular endothelial cells, mature and immature luminal epithelial cells, fibroblasts and myoepithelial cells were established from goat mammary gland biopsies. Their susceptibility to in vitro infection with two distinct types of CAEV was investigated by PCR, antigen expression and cytopathy. The capacity of infected mammary gland cells to bind uninfected caprine leukocytes was determined by flow cytometry. All cell types tested were susceptible to CAEV infection in vitro, with different levels of sensitivity according to cell phenotype. Our results suggest that the limited extent of natural infection of mammary gland cells reflects a protective local immune response, and that the myoepithelial cell could act as a reservoir cell. After infection, the mature luminal cell acquires the capacity to bind leukocytes in vitro, which could indicate a facilitation of cellular interactions. The distinct reactions of the different cell types to CAEV infection may be correlated with events leading to progressive lesion development during the natural infection. |
| Databáze: | OpenAIRE |
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