Mapping genetic variants underlying differences in the central nitrogen metabolism in fermenter yeasts
| Autor: | Francisco Salinas, Omayra Aguilera, Francisco A. Cubillos, Gianni Liti, Claudio Martínez, Matías Jara, Verónica García |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Heredity Genetic Linkage Nitrogen Nitrogen assimilation Science Population Quantitative Trait Loci Nitrogen Metabolism Wine Yeast and Fungal Models Saccharomyces cerevisiae Quantitative trait locus Biology Genetic analysis Biochemistry Model Organisms Pleiotropy Genetic Mutation Genetic variation Ammonium Compounds Genetics Genetic variability Amino Acids education Alleles Genetic diversity education.field_of_study Multidisciplinary Complex Traits Alcoholic Beverages Mutation Types Genetic Variation Membrane Proteins Amino Acid Transport Systems Neutral Metabolism Fermentation Medicine Research Article |
| Zdroj: | PLOS ONE Artículos CONICYT CONICYT Chile instacron:CONICYT PLoS ONE, Vol 9, Iss 1, p e86533 (2014) PLoS ONE |
| Popis: | Different populations within a species represent a rich reservoir of allelic variants, corresponding to an evolutionary signature of withstood environmental constraints. Saccharomyces cerevisiae strains are widely utilised in the fermentation of different kinds of alcoholic beverages, such as, wine and sake, each of them derived from must with distinct nutrient composition. Importantly, adequate nitrogen levels in the medium are essential for the fermentation process, however, a comprehensive understanding of the genetic variants determining variation in nitrogen consumption is lacking. Here, we assessed the genetic factors underlying variation in nitrogen consumption in a segregating population derived from a cross between two main fermenter yeasts, a Wine/European and a Sake isolate. By linkage analysis we identified 18 main effect QTLs for ammonium and amino acids sources. Interestingly, majority of QTLs were involved in more than a single trait, grouped based on amino acid structure and indicating high levels of pleiotropy across nitrogen sources, in agreement with the observed patterns of phenotypic co-variation. Accordingly, we performed reciprocal hemizygosity analysis validating an effect for three genes, GLT1, ASI1 and AGP1. Furthermore, we detected a widespread pleiotropic effect on these genes, with AGP1 affecting seven amino acids and nine in the case of GLT1 and ASI1. Based on sequence and comparative analysis, candidate causative mutations within these genes were also predicted. Altogether, the identification of these variants demonstrate how Sake and Wine/European genetic backgrounds differentially consume nitrogen sources, in part explaining independently evolved preferences for nitrogen assimilation and representing a niche of genetic diversity for the implementation of practical approaches towards more efficient strains for nitrogen metabolism. |
| Databáze: | OpenAIRE |
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