Nuclear envelope removal/maintenance determines the structural and functional remodelling of embryonic red blood cell nuclei in activated mouse oocytes
| Autor: | Ewa Borsuk, Renata Czolowska, Daniel Szöllösi, Pascale Debey, Maria S. Szöllösi |
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| Rok vydání: | 1998 |
| Předmět: |
Erythrocytes
Transcription Genetic Nuclear Envelope Nucleolus Somatic cell Parthenogenesis Hybrid Cells Biology Cell Fusion Mice Prophase medicine Animals Cells Cultured Female pronucleus Pronucleus Oocyte activation DNA Polymerase II Cell Biology Cell cycle Fetal Blood Chromatin Cell biology Mice Inbred C57BL Microscopy Electron Red blood cell medicine.anatomical_structure Mice Inbred CBA Oocytes Female Developmental Biology |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1469-8730 0967-1994 |
| DOI: | 10.1017/s0967199400005098 |
| Popis: | SummaryNuclei of embryonic red blood cells (e-RBC) from 12-day mouse fetuses are arrested in Gophase of the cell cycle and have low transcriptional activity. These nuclei were transferred with help of polyethylene glycol (PEG)-mediated fusion to parthenogenetically activated mouse oocytes and heterokaryons were analysed for nuclear structure and transcriptional activity. If fusion proceeded 25–45 min after oocyte activation, e-RBC nuclei were induced to nuclear envelope breakdown and partial chromatin condensation, followed by formation of nuclei structurally identical with pronuclei. These ‘pronuclei’, similar to egg (female) pronuclei, remained transcriptionally silent over several hours ofin vitroculture. If fusion was performed 1 h or later (up to 7 h) after activation, the nuclear envelope of e-RBC nuclei remained intact and nuclear remodelling was less spectacular (slight chromatin decondensation, formation of nucleolus precursor bodies). These nuclei, however, reinforced polymerase-II-dependent transcription within a few hours ofin vitroculture. Our present experiments, together with our previous work, demonstrate that nuclear envelope breakdown/maintenance are critical events for nuclear remodelling in activated mouse oocytes and that somatic dormant nuclei can be stimulated to renew transcription at a time when the female pronucleus remains transcriptionally silent. |
| Databáze: | OpenAIRE |
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