A soluble cadmium-binding component in rat and dog spleen
| Autor: | David E. Amacher, Keith L. Ewing |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
inorganic chemicals
Male Cytoplasm Sucrose chemistry.chemical_element Spleen law.invention chemistry.chemical_compound Dogs law Metalloproteins medicine Environmental Chemistry Animals Cadmium binding General Environmental Science Cadmium Chromatography Molecular mass Chemistry Public Health Environmental and Occupational Health Rats Molecular Weight medicine.anatomical_structure Solubility Sephadex Female Metallothionein Atomic absorption spectroscopy |
| Zdroj: | Archives of environmental health. 30(10) |
| ISSN: | 0003-9896 |
| Popis: | The spleens of cadmium-treated Sprague-Dawley rats and mongrel dogs were examined for cytoplasmic soluble cadmium-binding moieties following the cessation of parenteral cadmium exposure. Cell homogenates prepared in 0.25M sucrose were centrifuged at 29,000 g and the supernatant fractionated on a fractionating column (Sephadex G-75). Ultraviolet absorbing peaks that showed characteristically high 250-nm to 280-nm absorbences were further analyzed for cadmium content by atomic absorption spectrophotometry. Cadmium-binding fractions isolated from the spleens of both species were characteristically similar to rat kidney metallothionein-like substances isolated and identified by the same procedure. Molecular weights for the canine and rat spleen cadmium-binding components were estimated at 14,783 +/- 343 and 13,916 +/- 490, respectively. These preliminary data indicate the production and persistence of soluble metallothionein-like substances in spleen that bind and retain cadmium ions long after cadmium exposure has ceased. |
| Databáze: | OpenAIRE |
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