Analyzing cannabinoid-induced abnormal behavior in a zebrafish model
| Autor: | Ken-ichi Yoshida, Akihiro Hasumi, Hideyuki Maeda |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Life Cycles Light Physiology medicine.medical_treatment Social Sciences Pharmacology Larvae 0302 clinical medicine Medicine and Health Sciences Psychology Drug Interactions Zebrafish Multidisciplinary Behavior Animal Animal Behavior biology Pharmaceutics Chemistry Physics Electromagnetic Radiation Eukaryota Drugs Animal Models Experimental Organism Systems Osteichthyes Vertebrates Physical Sciences Darkness Medicine Abnormality Locomotion Research Article medicine.drug Science Period (gene) Danio Research and Analysis Methods 03 medical and health sciences Model Organisms Drug Therapy medicine Animals Inhibitory effect Behavior Cannabinoids Biological Locomotion Organisms Biology and Life Sciences Rodent model biology.organism_classification Fish 030104 developmental biology Fresh water Animal Studies Cannabinoid Zoology Cannabidiol 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | PLoS ONE, Vol 15, Iss 10, p e0236606 (2020) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | In this study, we investigated locomotor activity and responses to repeated light and dark stimuli to assess cannabinoid-induced abnormal behavior in zebrafish larvae (Danio rerio), as an alternative to standard rodent models. To induce the desired responses, we used cannabidiol and WIN55,212–2, two major cannabinoid components. A repeated light and dark test was used to assess how drug exposure influences locomotory responses. Larvae were examined after moderate cannabidiol and WIN55,212–2 exposure and at 24 h after transfer to untreated water. We found that cannabidiol did not produce a dose-dependent inhibitory effect on locomotor activity, with both 0.5 and 10 μg/mL concentrations reducing movement velocity and the total distance moved. However, 10 μg/mL cannabidiol was observed to attenuate the responses of larvae exposed to darkness. No differences were detected between the control and cannabidiol-treated groups after 24 h in fresh water. Fish treated with WIN55,212–2 at 0.5 and 1 μg/mL showed virtually no activity, even in darkness, whereas a concentration of 10 μg/mL induced mortality. A 24-h period in fresh water had the effect of reversing most of the drug-induced immobilization, even in the WIN55,212-2-treated groups. Larvae were also evaluated for their responses to cannabidiol subsequent to an initial exposure to WIN55,212–2, and it was accordingly found that treatment with cannabidiol could attenuate WIN55,212-2-induced abnormal immobilization, whereas equivalent doses of cannabidiol and WIN55,212–2 produced a mixed response. In conclusion, the behavioral effects of the two cannabinoids cannabidiol and WIN55,212–2 appear to be ratio dependent. Furthermore, the repeated light and dark test could serve as a suitable method for assaying drug-induced behavior. |
| Databáze: | OpenAIRE |
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