The role of artichoke leaf tincture (Cynara scolymus) in the suppression of DNA damage and atherosclerosis in rats fed an atherogenic diet
| Autor: | Jelena Kotur Stevuljević, Natasa Bogavac-Stanojevic, Miron Sopić, Janja Zupan, Zorica Vujić, Janja Marc, Miroslav Radenković, Zorana Jelic-Ivanovic, Darko Cerne, Jelena Munjas, Milkica Crevar-Sakač |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty DNA damage monocyte chemoattractant protein-1 Pharmaceutical Science Context (language use) oxidative damage 03 medical and health sciences chemistry.chemical_compound Rutin 0302 clinical medicine Chlorogenic acid Internal medicine Cynara scolymus Drug Discovery Oxidative damage Caffeic acid medicine Animals Rats Wistar 2. Zero hunger Pharmacology NADPH oxidase biology Cholesterol Plant Extracts lcsh:RM1-950 haemeoxygenase-1 Tincture General Medicine Atherosclerosis 3. Good health Rats Plant Leaves lcsh:Therapeutics. Pharmacology 030104 developmental biology Endocrinology Complementary and alternative medicine chemistry 030220 oncology & carcinogenesis biology.protein gene expression Molecular Medicine Diet Atherogenic DNA Damage |
| Zdroj: | Pharmaceutical Biology Pharmaceutical Biology, Vol 56, Iss 1, Pp 138-144 (2018) |
| Popis: | Context: Polyphenols and flavonoids in artichoke leaf tincture (ALT) protect cells against oxidative damage. Objectives: We examined ALT effects on deoxyribonucleic acid (DNA) damage and lipid profiles in rat plasma and gene expression in rat aorta [haemeoxygenase-1 (HO1), haemeoxygenase-2 (HO2), NADPH oxidase 4 (NOX-4), monocyte chemoattractant protein-1 (MCP-1) and nuclear factor (erythroid-derived 2)-like 2 (Nrf2)]. Materials and methods: Eighteen male Wistar albino rats were divided into three groups (n = 6/group): The control group (CG) was fed with standard pellet chow for 11 weeks; the AD group was fed for a similar period of time with pellet chow supplemented with 2% cholesterol, 3% sunflower oil and 1% sodium cholate. The ADA group was fed with pellet chow (for 1 week), the atherogenic diet (see above) for the following 4 weeks and then with ALT (0.1 mL/kg body weight) and atherogenic diet for 6 weeks. According to HPLC analysis, the isolated main compounds in ALT were chlorogenic acid, caffeic acid, isoquercitrin and rutin. Results: Normalized HO-1 [0.11 (0.04–0.24)] and MCP-1 [0.29 (0.21–0.47)] mRNA levels and DNA scores [12.50 (4.50–36.50)] were significantly lower in the ADA group than in the AD group [0.84 (0.35–2.51)], p = 0.021 for HO-1 [0.85 (0.61–3.45)], p = 0.047 for MCP-1 and [176.5 (66.50–221.25)], p = 0.020 for DNA scores. HO-1 mRNA was lower in the ADA group than in the CG group [0.30 (0.21–0.71), p = 0.049]. Conclusions: Supplementation with ALT limited the effects of the atherogenic diet through reduced MCP-1 expression, thereby preventing oxidative damage. |
| Databáze: | OpenAIRE |
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