Temporal dynamics of trematode intermediate snail host environmental DNA in small water body habitats
Autor: | Chelsea N. Davis, Fiona Tyson, Rhys Jones, Emma Davies, Hefin Wyn Williams, David Cutress, Dewi Llyr Jones, Michael T. Rose, Peter M. Brophy, Manod Williams |
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Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
Zoology Snail Trematode Infections 010603 evolutionary biology 01 natural sciences trematode 03 medical and health sciences biology.animal Fasciola hepatica Animals Environmental DNA Ecosystem fluke 030304 developmental biology Galba truncatula small water bodies 0303 health sciences biology Host (biology) Calicophoron daubneyi fungi Water biology.organism_classification environmental DNA DNA Environmental intermediate snail host Infectious Diseases Water body Habitat Animal Science and Zoology Parasitology Trematoda eDNA generalized estimation equation Research Article |
Zdroj: | Parasitology |
ISSN: | 1469-8161 |
Popis: | Environmental DNA (eDNA) surveying has potential to become a powerful tool for sustainable parasite control. As trematode parasites require an intermediate snail host that is often aquatic or amphibious to fulfil their lifecycle, water-based eDNA analyses can be used to screen habitats for the presence of snail hosts and identify trematode infection risk areas. The aim of this study was to identify climatic and environmental factors associated with the detection of Galba truncatula eDNA. Fourteen potential G. truncatula habitats on two farms were surveyed over a 9-month period, with eDNA detected using a filter capture, extraction and PCR protocol with data analysed using a generalized estimation equation. The probability of detecting G. truncatula eDNA increased in habitats where snails were visually detected, as temperature increased, and as water pH decreased (P < 0.05). Rainfall was positively associated with eDNA detection in watercourse habitats on farm A, but negatively associated with eDNA detection in watercourse habitats on farm B (P < 0.001), which may be explained by differences in watercourse gradient. This study is the first to identify factors associated with trematode intermediate snail host eDNA detection. These factors should be considered in standardized protocols to evaluate the results of future eDNA surveys. |
Databáze: | OpenAIRE |
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