Development of real-time PCR assay for the detection of Mycoplasma bovis
| Autor: | T.R. Arun, Praveen K. Gupta, D. Jena, Valsala Rekha, Dinesh Kumar, Sthita Pragnya Behera, Rajneesh Rana, Sonal |
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| Rok vydání: | 2018 |
| Předmět: |
Mycoplasma bovis
Bovine milk Buffaloes 040301 veterinary sciences Cattle Diseases Real-Time Polymerase Chain Reaction medicine.disease_cause Sensitivity and Specificity Cross-reactivity Microbiology 0403 veterinary science Food Animals medicine Animals Mycoplasma Infections Lung Gene biology 0402 animal and dairy science 04 agricultural and veterinary sciences Mycoplasma biology.organism_classification medicine.disease 040201 dairy & animal science Mastitis Milk Real-time polymerase chain reaction Cattle Female Animal Science and Zoology Bacteria |
| Zdroj: | Tropical Animal Health and Production. 50:875-882 |
| ISSN: | 1573-7438 0049-4747 |
| Popis: | Mycoplasma bovis is one of the important bovine mycoplasma involved in economically important clinical conditions like respiratory diseases, otitis media, and mastitis. The present study was undertaken with the objective of developing a SYBR Green dye-based real-time PCR assay targeting uvrC gene for the diagnosis of M. bovis. The analytical sensitivity and specificity of the assay were evaluated. The test showed 103-fold more sensitivity than conventional PCR and detected down to 100 fg level of DNA. It was found to be specific, as no cross reactivity was shown with other related bacteria and Mycoplasma species. The developed assay was able to detect down to 40 copies of uvrC gene from spiked bovine milk samples. At present, this developed assay may be used as a valuable diagnostic tool for the detection of Mycoplasma bovis. |
| Databáze: | OpenAIRE |
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