Immunodominance with progenitor B cell diversity in the neutralizing antibody repertoire to influenza infection

E, whereas changes at either HA1 158 G-->E or HA1 198 A-->E are selected for by mAb from BALB.K (H-2k) donors. The structural basis for immunodominance, and potential diversity of progenitor B cells, was investigated by sequence analysis of H and L chain gene rearrangements in mAb specific for HA1 158 or HA1 198. No correlation was found between antibody specificity and VH or VL gene usage, and a minimum of three to six progenitor cells contributed to the individual's repertoire for a single antigenic site. However, in a further analysis of the HA1 158-specific antibody response of CBA/Ca (H-2k) donors, there was highly restricted light chain gene usage. Focusing of the immune repertoire to limited regions of the HA molecule during a primary viral infection may be a significant factor in immune pressure for antigenic variation, particularly since there is no evident restriction in the antibody response to immunization. -->

ISSN:	1521-4141 0014-2980
Přístupová URL adresa:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc6bc112fc68b94fedffd7d1c4105d17 https://doi.org/10.1002/eji.1830250702
Rights:	CLOSED
Přírůstkové číslo:	edsair.doi.dedup.....bc6bc112fc68b94fedffd7d1c4105d17
Autor:	A C Patera, Christine M. Graham, C A Smith, D. B. Thomas
Rok vydání:	1995
Předmět:	viruses Molecular Sequence Data Immunology Immunoglobulin Variable Region Hemagglutinins Viral Hemagglutinin (influenza) Immunodominance Biology Antibodies Viral Major histocompatibility complex Mice Antigen Neutralization Tests Antigenic variation Animals Immunology and Allergy Neutralizing antibody Antigens Viral DNA Primers B-Lymphocytes Mice Inbred BALB C Base Sequence Genes Immunoglobulin H-2 Antigens Antibodies Monoclonal Antibody Diversity Virology Molecular biology Protein Structure Tertiary Immunoglobulin Isotypes Multigene Family Mice Inbred CBA biology.protein Immunoglobulin Light Chains Antibody Immunoglobulin Heavy Chains Epitope Mapping
Zdroj:	European Journal of Immunology. 25:1803-1809
ISSN:	1521-4141 0014-2980
Popis:	We report striking immunodominance in the neutralizing antibody responses of major histocompatibility complex congenic mice to natural infection with influenza virus (H3N2 subtype), as deduced by sequencing the hemagglutinin (HA) genes of monoclonal antibody (mAb)-selected mutant viruses. A majority of mAb, established from individual BALB/c (H-2d) mice, select mutant viruses containing the same single amino acid substitution in the membrane distal ecto-domain, HA1 198 A-->E, whereas changes at either HA1 158 G-->E or HA1 198 A-->E are selected for by mAb from BALB.K (H-2k) donors. The structural basis for immunodominance, and potential diversity of progenitor B cells, was investigated by sequence analysis of H and L chain gene rearrangements in mAb specific for HA1 158 or HA1 198. No correlation was found between antibody specificity and VH or VL gene usage, and a minimum of three to six progenitor cells contributed to the individual's repertoire for a single antigenic site. However, in a further analysis of the HA1 158-specific antibody response of CBA/Ca (H-2k) donors, there was highly restricted light chain gene usage. Focusing of the immune repertoire to limited regions of the HA molecule during a primary viral infection may be a significant factor in immune pressure for antigenic variation, particularly since there is no evident restriction in the antibody response to immunization.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc6bc112fc68b94fedffd7d1c4105d17 https://doi.org/10.1002/eji.1830250702 Zobrazit plný text záznamu Plný text