Enzyme-Based Test Strips for Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard
| Autor: | Martin Stepan, Zbynek Prokop, Kamila Vymazalova, Michael Gruber, Šárka Bidmanová, Mark-Steven Steiner, Axel Duerkop, Otto S. Wolfbeis, Jiri Damborsky |
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| Rok vydání: | 2016 |
| Předmět: |
Chromatography
010405 organic chemistry Chemistry 010401 analytical chemistry Sulfur mustard 01 natural sciences Rapid detection 0104 chemical sciences Analytical Chemistry Test strips chemistry.chemical_compound Color changes Chemical agents Degradation (geology) Sample preparation Haloalkane dehalogenase |
| Zdroj: | Analytical Chemistry. 88:6044-6049 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.6b01272 |
| Popis: | Sulfur mustard is a chemical agent of high military and terroristic significance. No effective antidote exists, and sulfur mustard can be fairly easily produced in large quantity. Rapid field testing of sulfur mustard is highly desirable. Existing analytical devices for its detection are available but can suffer from low selectivity, laborious sample preparation, and/or the need for complex instrumentation. We describe a new kind of test strip for rapid detection of gaseous sulfur mustard that is based on its degradation by the enzyme haloalkane dehalogenase that is accompanied by a change of local pH. This change can be detected using pH indicators contained in the strips whose color changes from blue-green to yellow within 10 min. In addition to visual read-out, we also demonstrate quantitative reflectometric readout by using a conventional digital camera based on red-green-blue data acquisition. Organic haloalkanes, such as 1,2-dichloroethane, have a negligible interfering effect. The visual limit of detection is 20 μg/L, and the one for red-green-blue read-out is as low as 3 μg/L. The assays have good reproducibility ±6% and ±2% for interday assays and intraday assays, respectively. The strips can be stored for at least 6 months without loss of function. They are disposable and can be produced fairly rapidly and at low costs. Hence, they represent a promising tool for in-field detection of sulfur mustard. |
| Databáze: | OpenAIRE |
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