REGULATORY ELEMENTS CONTROLLING CI-MSXB TISSUE-SPECIFIC EXPRESSION DURING CIONA INTESTINALIS EMBRYONIC DEVELOPMENT
| Autor: | Margherita Branno, Monia Teresa Russo, Alessandro Amoroso, Aldo Donizetti, Salvatore D'Aniello, Laura Fucci, Annamaria Locascio, Francesco Aniello |
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| Přispěvatelé: | Russo, Mt, Donizetti, Aldo, Locascio, A, D'Aniello, S, Amoroso, A, Aniello, Francesco, Fucci, Laura, Branno, M. |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Nervous system
Molecular Sequence Data Electrophoretic Mobility Shift Assay Regulatory Sequences Nucleic Acid Biology 03 medical and health sciences 0302 clinical medicine Genes Reporter Cis-element Gene expression Animals Homeobox Gene family Ciona intestinalis LacZ reporter gene Molecular Biology Transcription factor Gene 030304 developmental biology Homeodomain Proteins Genetics 0303 health sciences Base Sequence Electroporation Gene Expression Regulation Developmental Promoter Cell Biology biology.organism_classification Blotting Southern Italy Lac Operon Neurula Pharynx 030217 neurology & neurosurgery Transcription Factors Developmental Biology |
| Popis: | The msh/Msx family is a subclass of homeobox-containing genes suggested to perform a conserved function in the patterning of the early embryo. We had already isolated a member of this gene family (Ci-msxb) in Ciona intestinalis, which has a very complex expression pattern during embryogenesis. To identify the regulatory elements controlling its tissue-specific expression, we have characterized the gene structure and the regulatory upstream region. By electroporation experiments, we demonstrated that a 3.8-kb region located upstream of the gene contains all the regulatory elements able to reproduce its spatial expression pattern. Analyzing progressively truncated fragments of this region, three discrete and separate regions driving LacZ reporter gene expression in the ventral epidermis, primordial pharynx and neural territories have been identified. We further investigated the element(s) necessary for Ci-msxb activation in the nervous system during embryonic development by in vivo and in vitro experiments. Both electroporation and gel-shift assays of overlapping wild type and mutated oligonucleotides demonstrated that a unique sequence of 30 bp is involved in Ci-msxb neural activation from neurula to larva stage. This sequence contains consensus binding sites for various ubiquitous transcription factors such as TCF11 whose possible implication in formation of the regulatory complexes is discussed. |
| Databáze: | OpenAIRE |
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