Picazoplatin, an Azide-Containing Platinum(II) Derivative for Target Analysis by Click Chemistry
| Autor: | Jonathan D. White, Maire F. Osborn, Victoria J. DeRose, Michael M. Haley, Lindsay E. Guzman, Alan D. Moghaddam |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Models
Molecular Azides Organoplatinum Compounds Oligonucleotide Stereochemistry Drug discovery Oligonucleotides General Chemistry Biochemistry Combinatorial chemistry Catalysis Article Picoplatin chemistry.chemical_compound Colloid and Surface Chemistry chemistry Alkynes Drug Discovery Click chemistry Moiety Click Chemistry Azide DNA Derivative (chemistry) Fluorescent Dyes |
| Popis: | Despite the broad use of platinum-based chemotherapeutics, identification of their full range of cellular targets remains a significant challenge. In order to identify, visualize, and isolate cellular targets of Pt(II) complexes, we have modified the chemotherapeutic drug picoplatin with an azide moiety for subsequent click reactivity. The new compound picazoplatin readily binds DNA and RNA oligonucleotides and undergoes facile post-labeling click reactions to alkyne-fluorophore conjugates. Pt-fluorophore click reactions in rRNA purified from drug-treated Saccharomyces cerevisiae demonstrate its potential for future in vivo efforts. |
| Databáze: | OpenAIRE |
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