Selection system for genes encoding nuclear-targeted proteins
| Autor: | Teruhisa Noguchi, Tamaki Oda, Maiko Kondo, Nobuhide Ueki, Masaaki Muramatsu, Kazuhiro Yano |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
DNA
Complementary Nuclear Localization Signals Biomedical Engineering Bioengineering Computational biology Biology Applied Microbiology and Biotechnology Complementary DNA Animals Humans Amino Acid Sequence Cloning Molecular Nuclear protein Gene DNA Primers Cell Nucleus Genetics Base Sequence cDNA library Brain Nuclear Proteins Fusion protein COS Cells Molecular Medicine Nuclear transport Function (biology) Nuclear localization sequence Biotechnology |
| Zdroj: | Nature Biotechnology. 16:1338-1342 |
| ISSN: | 1546-1696 1087-0156 |
| Popis: | Nuclear proteins have essential roles in cell proliferation and differentiation. We have developed a yeast selection system-the nuclear transportation trap (NTT)-to identify genes encoding nuclear transport signals. Both unknown and previously identified nuclear localization signals were identified from a human fetal brain cDNA library. The majority (75%) of the unknown proteins examined were exclusively localized to the nucleus in COS-7 cells. We propose that NTT is an efficient method for isolating cDNAs that encode nuclear targeted proteins that can be applied to the retrieval of novel nuclear proteins and to annotate gene function. |
| Databáze: | OpenAIRE |
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