Structural Basis for the Slow Dark Recovery of a Full-Length LOV Protein from Pseudomonas putida
| Autor: | Ulrich Krauss, Karl-Erich Jaeger, Joachim Granzin, Dieter Willbold, Renu Batra-Safferling, Franco Circolone, Katrin Jentzsch, Thomas Drepper |
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| Rok vydání: | 2012 |
| Předmět: |
Light
biology Protein family Flavin Mononucleotide Pseudomonas putida Mutagenesis Protein Data Bank (RCSB PDB) Flavin mononucleotide Crystallography X-Ray Photoreceptors Microbial biology.organism_classification Protein Structure Tertiary Adduct chemistry.chemical_compound Protein structure Bacterial Proteins Biochemistry chemistry Structural Biology PAS domain Biophysics Molecular Biology |
| Zdroj: | Journal of Molecular Biology. 417:362-374 |
| ISSN: | 0022-2836 |
| Popis: | Blue-light photoreceptors containing light–oxygen–voltage (LOV) domains regulate a myriad of different physiological responses in both eukaryotes and prokaryotes. Their light sensitivity is intricately linked to the photochemistry of the non-covalently bound flavin mononucleotide (FMN) chromophore that forms a covalent adduct with a conserved cysteine residue in the LOV domain upon illumination with blue light. All LOV domains undergo the same primary photochemistry leading to adduct formation; however, considerable variation is found in the lifetime of the adduct state that varies from seconds to several hours. The molecular mechanism underlying this variation among the structurally conserved LOV protein family is not well understood. Here, we describe the structural characterization of PpSB1-LOV, a very slow cycling full-length LOV protein from the Gram-negative bacterium Pseudomonas putida KT2440. Its crystal structure reveals a novel dimer interface that is mediated by N- and C-terminal auxiliary structural elements and a unique cluster of four arginine residues coordinating with the FMN-phosphate moiety. Site-directed mutagenesis of two arginines (R61 and R66) in PpSB1-LOV resulted in acceleration of the dark recovery reaction approximately by a factor of 280. The presented structural and biochemical data suggest a direct link between structural features and the slow dark recovery observed for PpSB1-LOV. The overall structural arrangement of PpSB1-LOV, together with a complementary phylogenetic analysis, highlights a common ancestry of bacterial LOV photoreceptors and Per-ARNT-Sim chemosensors. |
| Databáze: | OpenAIRE |
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