Binding of the apo and holo forms of the serum vitamin D-binding protein to human lymphocyte cytoplasm and membrane by indirect immunofluorescence
| Autor: | Jacques Constans, Françoise Oksman, Michel Viau |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Cytoplasm
Vitamin D-binding protein Lymphocyte Immunology Fluorescent Antibody Technique Biology medicine Vitamin D and neurology Animals Humans Immunology and Allergy Lymphocytes Vitamin D Active metabolite Goats Binding protein Cell Membrane Molecular biology In vitro Membrane medicine.anatomical_structure Biochemistry Rabbits Carrier Proteins circulatory and respiratory physiology |
| Zdroj: | Immunology Letters. 3:159-162 |
| ISSN: | 0165-2478 |
| DOI: | 10.1016/0165-2478(81)90120-6 |
| Popis: | The indirect immunofluorescence method is used to study the binding of the serum vitamin D carrier protein (DBP) to lymphocytes. It is shown that in vitro this serum protein will bind to the lymphocyte cytoplasm and intact membrane. This result is in agreement with recent evidence of an actin-binding protein present in the serum. Besides, a difference in the binding to the lymphocyte membrane is observed between the holo forms of the DBP with the different vitamin D derivatives. These findings could be relevant in illuminating the possible role of the DBP in the cellular metabolism of the active metabolites of vitamin D and in the cellular mobility. |
| Databáze: | OpenAIRE |
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