Early Detection of Response to Experimental Chemotherapeutic Top216 with [18F]FLT and [18F]FDG PET in Human Ovary Cancer Xenografts in Mice
| Autor: | Kamille Dumong Erichsen, Peter Buhl Jensen, Mette Munk Jensen, Fredrik Björkling, Maxwell Sehested, Jacob Madsen, Andreas Kjaer, Liselotte Højgaard |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Radiology and Medical Imaging Urology Oncology/Oncology Agents Early detection lcsh:Medicine Mice Nude Antineoplastic Agents 18f fdg pet Mice In vivo Fluorodeoxyglucose F18 Chemotherapy effects Medicine Animals Humans lcsh:Science Cell Proliferation Ovarian Neoplasms Multidisciplinary Tumor size medicine.diagnostic_test business.industry lcsh:R Parallel study Ovary cancer Xenograft Model Antitumor Assays Dideoxynucleosides Disease Models Animal Oncology Positron emission tomography Positron-Emission Tomography Radiology and Medical Imaging/PET and SPECT Imaging lcsh:Q Female Drug Monitoring Radiopharmaceuticals business Nuclear medicine Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 5, Iss 9, p e12965 (2010) |
| ISSN: | 1932-6203 |
| Popis: | BACKGROUND: 3'-Deoxy-3'-[(18)F]fluorothymidine ((18)F-FLT) is a tracer used to assess cell proliferation in vivo. The aim of the study was to use (18)F-FLT positron emission tomography (PET) to study treatment responses to a new anti-cancer compound. To do so, we studied early anti-proliferative effects of the experimental chemotherapy Top216 non-invasively by PET. METHODOLOGY/PRINCIPAL FINDINGS: In vivo uptake of (18)F-FLT in human ovary cancer xenografts in mice (A2780) was studied at various time points after Top216 treatment (50 mg/kg i.v. at 0 and 48 hours) was initiated. Baseline (18)F-FLT scans were made before either Top216 (n = 7-10) or vehicle (n = 5-7) was injected and repeated after 2 and 6 hours and 1 and 5 days of treatment. A parallel study was made with 2'-deoxy-2'-[(18)F]fluoro-D-glucose ((18)F-FDG) (n = 8). Tracer uptake was quantified using small animal PET/CT. Imaging results were validated by tumor volume changes and gene-expression of Ki67 and TK1. Top216 (50 mg/kg 0 and 48 hours) inhibited the growth of the A2780 tumor compared to the control group (P |
| Databáze: | OpenAIRE |
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