Oral Biofilm Architecture on Natural Teeth
| Autor: | John E. Degener, M. Barbara M. van Leeuwen, Rudolf Gmür, Vincent Zijnge, Frank Abbas, Thomas Thurnheer, Hermie J. M. Harmsen |
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| Přispěvatelé: | Man, Biomaterials and Microbes (MBM), Groningen Institute for Gastro Intestinal Genetics and Immunology (3GI) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
DENTAL PLAQUE
lcsh:Medicine SEQUENCE DATA Dental Caries Dental plaque HUMAN PERIODONTAL POCKETS Bacterial Adhesion Microbiology Infectious Diseases/Bacterial Infections Candida albicans medicine Actinomyces Humans Tannerella forsythia Periodontitis lcsh:Science CLINICAL SPECIMENS In Situ Hybridization Fluorescence Phylogeny LASER-SCANNING MICROSCOPY Mouth FUSOBACTERIUM-NUCLEATUM Multidisciplinary TARGETED OLIGONUCLEOTIDE PROBES Bacteria Fusobacterium nucleatum biology ACTINOMYCES-NAESLUNDII lcsh:R Biofilm Microbiology/Medical Microbiology Streptococcus Tooth surface IN-SITU HYBRIDIZATION biology.organism_classification medicine.disease DIRECT VISUALIZATION Lactobacillus stomatognathic diseases Biofilms Host-Pathogen Interactions Actinomyces naeslundii lcsh:Q Ecology/Ecosystem Ecology Synergistetes Tooth Research Article |
| Zdroj: | PLoS ONE, Vol 5, Iss 2, p e9321 (2010) PLoS ONE PLoS ONE, 5(2):9321. PUBLIC LIBRARY SCIENCE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0009321 |
| Popis: | Periodontitis and caries are infectious diseases of the oral cavity in which oral biofilms play a causative role. Moreover, oral biofilms are widely studied as model systems for bacterial adhesion, biofilm development, and biofilm resistance to antibiotics, due to their widespread presence and accessibility. Despite descriptions of initial plaque formation on the tooth surface, studies on mature plaque and plaque structure below the gum are limited to landmark studies from the 1970s, without appreciating the breadth of microbial diversity in the plaque. We used fluorescent in situ hybridization to localize in vivo the most abundant species from different phyla and species associated with periodontitis on seven embedded teeth obtained from four different subjects. The data showed convincingly the dominance of Actinomyces sp., Tannerella forsythia, Fusobacterium nucleatum, Spirochaetes, and Synergistetes in subgingival plaque. The latter proved to be new with a possibly important role in host-pathogen interaction due to its localization in close proximity to immune cells. The present study identified for the first time in vivo that Lactobacillus sp. are the central cells of bacterial aggregates in subgingival plaque, and that Streptococcus sp. and the yeast Candida albicans form corncob structures in supragingival plaque. Finally, periodontal pathogens colonize already formed biofilms and form microcolonies therein. These in vivo observations on oral biofilms provide a clear vision on biofilm architecture and the spatial distribution of predominant species. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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