The yeast UASG is a transcriptional enhancer in human HeLa cells in the presence of the GAL4 trans-activator
| Autor: | Stephen Green, Pierre Chambon, Melvyn Hollis, Jia Rui Jin, Nicholas J. G. Webster |
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| Rok vydání: | 1988 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Transcription Genetic TATA box CAAT box DNA Recombinant Enhancer RNAs Simian virus 40 Biology Regulatory Sequences Nucleic Acid Transfection General Biochemistry Genetics and Molecular Biology Fungal Proteins Upstream activating sequence Enhancer trap Animals Humans Simplexvirus Enhancer DNA Fungal Promoter Regions Genetic Transcription factor fungi Promoter Estrogens Molecular biology Globins DNA-Binding Proteins Enhancer Elements Genetic Receptors Estrogen Rabbits HeLa Cells Plasmids Thymidine Transcription Factors |
| Zdroj: | Cell. 52(2) |
| ISSN: | 0092-8674 |
| Popis: | The yeast trans-activator protein GAL4, when expressed in HeLa cells, stimulates transcription from several class B (II) eukaryotic promoters containing GAL4 binding sites either as the full UASG or as synthetic 17-mers. The characteristics of this activation are indistinguishable from those of the SV40 enhancer. Transcription was similarly stimulated from either complex promoter regions containing multiple upstream elements or from a simple promoter region composed of only a TATA box. Addition of a 17-mer GAL4 binding site to the SV40 enhancer resulted in a synergistic enhancement of transcription in the presence of GAL4. Furthermore, chimeras of the human estrogen receptor DNA binding domain and either GAL4 or GCN4 activating "acidic" regions can activate a promoter region controlled by an estrogen-responsive enhancer. Together, these data indicate that the molecular mechanisms responsible for transcriptional enhancement have been conserved from yeast to man. |
| Databáze: | OpenAIRE |
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