Saliva Pooling Strategy for the Large-Scale Detection of SARS-CoV-2, Through Working-Groups Testing of Asymptomatic Subjects for Potential Applications in Different Workplaces
| Autor: | A.B. Benitez-Trinidad, K.J.G. Díaz-Resendiz, Ansonny Jhovanny Ojeda-Durán, G.A. Toledo-Ibarra, Ulises Mercado-Salgado, G.H. Ventura-Ramón, Manuel Iván Girón-Pérez, Daniel Alberto Girón Pérez, Rocío Alejandra Ruiz-Manzano, C.E. Covantes-Rosales |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Saliva Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Pooling Sensitivity and Specificity Asymptomatic Specimen Handling COVID-19 Testing stomatognathic system Internal medicine medicine Humans Sampling (medicine) Workplace Asymptomatic Infections Test sample Cycle threshold asyntomatic subjects saliva pools SARS-CoV-2 business.industry fungi COVID-19 prevention Public Health Environmental and Occupational Health COVID-19 COVID-19 Nucleic Acid Testing RNA Viral Original Article Contact Tracing medicine.symptom business Personal protection equipment |
| Zdroj: | Journal of Occupational and Environmental Medicine |
| ISSN: | 1536-5948 1076-2752 |
| Popis: | OBJECTIVE: To perform an improved large-scale SARS-CoV-2 detection on pooled tests of asymptomatic workers. METHODS: qRT-PCR validation of the SARS-CoV-2 detection in salivae samples and saliva pools and working-group saliva pooling and testing for SARS-CoV-2. RESULTS: We found a high Cycle threshold (Ct) correlation (râ=â0.9099) between swabs and saliva samples. Then, through the pooling strategy, we detected that 18/360 (5%) of individual saliva samples were SARS-CoV-2 positive. Saliva-pooling efficiency (360 of test sample/30 individual PCR) was higher (5.45) than the reported for swabbing group-testing and we spared 82% of the PCR reagents as well as sampling and personal protection equipment. CONCLUSION: Through this simplified and less expensive procedure, we detected in a short time asymptomatic-infected SARS-CoV-2-carriers that were isolated from their co-workers, thus, this methodology can be implemented in different workplaces to ensure consumers that employees are not infectious. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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