Measuring local properties inside a cell‐mimicking structure using rotating optical tweezers
| Autor: | Halina Rubinsztein-Dunlop, Lachlan J. Gibson, Shu Zhang, Timo A. Nieminen, Alexander B. Stilgoe |
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| Rok vydání: | 2019 |
| Předmět: |
Liposome
Materials science Optical Tweezers Rotation Cell Survival 010401 analytical chemistry General Engineering General Physics and Astronomy High resolution Nanotechnology General Chemistry 01 natural sciences General Biochemistry Genetics and Molecular Biology 0104 chemical sciences 010309 optics Membrane Optical tweezers Rheology Biomimetics Liposomes 0103 physical sciences Particle General Materials Science Lipid vesicle |
| Zdroj: | Journal of Biophotonics. 12 |
| ISSN: | 1864-0648 1864-063X |
| DOI: | 10.1002/jbio.201900022 |
| Popis: | Exploring the rheological properties of intracellular materials is essential for understanding cellular and subcellular processes. Optical traps have been widely used for physical manipulation of micro- and nano-objects within fluids enabling studies of biological systems. However, experiments remain challenging as it is unclear how the probe particle's mobility is influenced by the nearby membranes and organelles. We use liposomes (unilamellar lipid vesicles) as a simple biomimetic model of living cells, together with a trapped particle rotated by optical tweezers to study mechanical and rheological properties inside a liposome both theoretically and experimentally. Here we demonstrate that this system has the capacity to predict the hydrodynamic interaction between three-dimensional (3D) spatial membranes and internal probe particles within submicron distances, and it has the potential to aid in the design of high resolution optical micro/nanorheology techniques to be used inside living cells. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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