Diagnostic accuracy of self-collected vaginal samples for HPV DNA detection in women from South India
| Autor: | Nurul Ameen, Sasidharanpillai Sabeena, Santhosh Kuriakose, Jazeel Abdulmajeed, Nagaraja Ravishankar, Bindu Vijaykumar, Damodaran Binesh, Amrutha Ramachandran |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
medicine.medical_specialty India Uterine Cervical Neoplasms Diagnostic accuracy Gynecologic oncology Polymerase Chain Reaction Human Papillomavirus DNA Tests Specimen Handling 03 medical and health sciences 0302 clinical medicine Obstetrics and gynaecology Predictive Value of Tests Prevalence Medicine Humans 030212 general & internal medicine Papillomaviridae Early Detection of Cancer Cervical cancer 030219 obstetrics & reproductive medicine business.industry Obstetrics Papillomavirus Infections Obstetrics and Gynecology General Medicine Gold standard (test) Middle Aged medicine.disease Confidence interval HPV DNA detection Cross-Sectional Studies Female Sample collection business |
| Zdroj: | International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and ObstetricsREFERENCES. 149(2) |
| ISSN: | 1879-3479 |
| Popis: | Objectives: To assess the efficacy of self-collected vaginal samples compared with physician-collected cervical samples for the detection of HPVDNA. Methods: A hospital-based cross-sectional study was carried out among patients with newly diagnosed cervical cancer attending the Gynecologic Oncology Division, Department of Obstetrics and Gynecology and Radiation Oncology Department at Government Medical College, Kozhikode, Kerala between March 2017 and April 2019. Consenting patients collected their vaginal samples, followed by cervical sample collection by the clinician. The paired samples were transported at 4–8 °C to the laboratory. Amplification of LCR/E6/E7 regions of the HPV genome was done by polymerase chain reaction (PCR). The agreement level between paired samples was assessed by the Kappa index Results: Among the 114 cervical cancer patients enrolled in the present cross-sectional study, the prevalence of HPV DNA was 78.1% (95% confidence interval [CI] 69.2%– 85%) in cervical samples and 77.2% in vaginal samples (95% CI 68.7%–83.9%). The overall agreement between the two sampling methods was 93.9% and the kappa value was 0.82 (P |
| Databáze: | OpenAIRE |
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